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Gelite™ Orange Nucleic Acid Gel Staining Kit

160 ng of 1 kb Plus DNA Ladder  (ThermoFisher 10787018) in 0.9% agarose/TBE electrophoresis gel were stained with Gelite™ Orange and SYBR® Gold, and imaged with 254-nm UV transilluminator using UVP Bioimaging System.
160 ng of 1 kb Plus DNA Ladder  (ThermoFisher 10787018) in 0.9% agarose/TBE electrophoresis gel were stained with Gelite™ Orange and SYBR® Gold, and imaged with 254-nm UV transilluminator using UVP Bioimaging System.
Ordering information
Price ()
Catalog Number17594
Unit Size
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Additional ordering information
Telephone1-408-733-1055
Fax1-408-733-1304
Emailsales@aatbio.com
InternationalSee distributors
ShippingStandard overnight for United States, inquire for international
Storage, safety and handling
H-phraseH303, H313, H340
Hazard symbolT
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R68
UNSPSC41116134

OverviewpdfSDSpdfProtocol


Gelite™ Orange is an extremely sensitive nucleic acid gel stain for detecting DNA or RNA in gels using a standard 300 nm UV transilluminator and Polaroid 667 black-and-white print film. As with Helixyte™ Green stain, this remarkable sensitivity can be attributed to a combination of unique dye characteristics. Because the nucleic acid-bound Gelite™ Orange dye exhibits excitation maxima at both ~495 nm and ~300 nm (the emission maximum is ~537 nm), it is compatible with a wide variety of instrumentation, ranging from UV epi- and transilluminators and blue-light transilluminators, to mercury-arc lamp- and argon-ion laser-based gel scanners. Our Gelite™ Orange Nucleic Acid Gel Staining Gel Kit includes our Gelite™ Orange nucleic acid stain with an optimized and robust protocol. It provides a convenient solution for staining nucleic acid samples in gels.

Platform


Transilluminator

Excitation254 nm or 300 nm
EmissionLong path green filter (ex. SYBR or GelStar)

Components


Component A: Gelite™ Orange Stain1 vial (20 µL)
Component B: Gel Loading Buffer3 x 1 mL

Example protocol


PREPARATION OF WORKING SOLUTION

Add 1 μL of Gelite™ Orange (Component A) into 200 μL of 5X Gel Loading Buffer (Component B). Protect the working solution from light by covering it with foil or placing it in the dark.

SAMPLE EXPERIMENTAL PROTOCOL

  1. Prepare DNA samples as you desired.
  2. Add 4 µL of Gelite™ Orange working solution into 16 µL of DNA samples and mix well. Incubate at room temperature for 5 - 15 minutes prior to electrophoresis.
  3. Run gels based on your standard protocol.
  4. Image the gel with a 300 nm ultraviolet or 254 nm transilluminator, or a laser-based gel scanner using a long path green filter such as a SYBR® filter or GelStar® filter. 

References


View all 79 references: Citation Explorer
SYBR green real-time PCR for the detection of all enterovirus-A71 genogroups
Authors: Dubot-Peres A, Tan CY, de Chesse R, Sibounheuang B, Vongsouvath M, Phommasone K, Bessaud M, Gazin C, Thirion L, Phetsouvanh R, Newton PN, de Lamballerie X.
Journal: PLoS One (2014): e89963
Development of a SYBR Green I based one-step real-time PCR assay for the detection of Hantaan virus
Authors: Jiang W, Wang PZ, Yu HT, Zhang Y, Zhao K, Du H, Bai XF.
Journal: J Virol Methods (2014): 145
A Broadly Reactive One-Step SYBR Green I Real-Time RT-PCR Assay for Rapid Detection of Murine Norovirus
Authors: Hanaki K, Ike F, Kajita A, Yasuno W, Yanagiba M, Goto M, Sakai K, Ami Y, Kyuwa S.
Journal: PLoS One (2014): e98108
A SYBR Green I based real time RT-PCR assay for specific detection and quantitation of Peste des petits ruminants virus
Authors: Abera T, Thangavelu A, Joy Ch and ran ND, Raja A.
Journal: BMC Vet Res (2014): 22
A SYBR-green I quantitative real-time reverse transcription-PCR assay for rabies viruses with different virulence
Authors: Wang L, Liu Y, Zhang S, Wang Y, Zhao J, Miao F, Hu R.
Journal: Virol Sin (2014): 131
Novel strategy combining SYBR Green I with carbon nanotubes for highly sensitive detection of Salmonella typhimurium DNA
Authors: Mao P, Ning Y, Li W, Peng Z, Chen Y, Deng L.
Journal: Enzyme Microb Technol (2014): 15
Detection and characterization of Leishmania (Leishmania) and Leishmania (Viannia) by SYBR green-based real-time PCR and high resolution melt analysis targeting kinetoplast minicircle DNA
Authors: Ceccarelli M, Galluzzi L, Migliazzo A, Magnani M.
Journal: PLoS One (2014): e88845
Molecular detection of human rhinoviruses in respiratory samples: a comparison of Taqman probe-, SYBR green I- and BOXTO-based real-time PCR assays
Authors: Dupouey J, Ninove L, Ferrier V, Py O, Gazin C, Thirion-Perrier L, de Lamballerie X.
Journal: Virol J (2014): 31
Development of SYBR Green based real time PCR assay for detection of monodon baculovirus in Penaeus monodon
Authors: Ramesh Kumar D, Sanjuktha M, Rajan JJ, An and a Bharathi R, Santiago TC, Alav and i SV, Poornima M.
Journal: J Virol Methods (2014): 81
Development of a SYBR Green real-time RT-PCR assay for the detection of avian encephalomyelitis virus
Authors: Liu Q, Yang Z, Hao H, Cheng S, Fan W, Du E, Xiao S, Wang X, Zhang S.
Journal: J Virol Methods (2014): 46