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AAT Bioquest

What are the advantages of using fluorescent dyes to label cells?

Posted January 16, 2024


Answer

Fluorescent dyes are biological molecules that are made up of at least one fluorophore, which is a molecule that emits light on excitation by light energy. 

  • Offer higher specificity and sensitivity
  • Can be used to label live as well as fixed cells
  • Can be used to localize and track specific cellular components
  • Photostable and long-lasting
  • Can be multiplexed for labeling multiple targets simultaneously 
  • Can be used for quantitative analysis
  • Compatible with multiple imaging techniques
Additional resources

Fluorescent labeling and modification of proteins

Fluoresceins

iFluor® 488 succinimidyl ester