AAT Bioquest

What are the advantages of using secondary antibodies?

Posted June 30, 2022


Secondary antibodies are designed to recognize and bind to primary antibodies in a number of immunoassays. Using secondary antibodies in immunoassays offers several advantages.    

  • The signal amplification resulting from multiple secondary antibodies binding to a single primary antibody attracts additional reporter molecules to the antibody-antigen complex, which increases sensitivity.
  • A given secondary antibody is able to bind to any primary antibody of the same type and host species, which makes secondary antibodies more versatile.
  • Secondary antibodies expand conjugate options by offering extended access to a wider range of probes and increasing flexibility in assay design.
  • Prevents interference with the paratope or the primary antibody’s antigen-binding site and preserves the primary antibody’s activity. 
  • Secondary antibodies enable multiplexing or multi-labeling in experiments.
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