Lyases catalyze reactions in which chemical groups are either added to a substrate or removed from it, resulting in the creation of new bonds and alterations in the structure of the molecule.
Lyases play crucial roles in various metabolic pathways including the tricarboxylic acid (TCA) cycle, contributing to processes essential for intermediary metabolism and energy production.
Many lyases catalyze condensation or dehydration reactions in which they eliminate water molecules while breaking or forming breaking chemical bonds.
Lyases function independently of water molecules, unlike hydrolases, which depend on the presence of water in their catalytic process.
Lyases may utilize coenzymes such as pyridoxal phosphate to aid in their catalytic functions.