Basis of differentiation | Fluorescence | Luminescence |
Definition | Fluorescence is the absorption of energy by atoms or molecules followed by the emission of light or electromagnetic radiation | Is a spontaneous emission of light by a substance not resulting from heat |
Types | TIRF, wide-field epifluorescence microscopy, and confocal microscopy | Photoluminesce, chemiluminescence, crystalloluminescence |
Lifetime | The lifetime of the excited state is very short, and photons are emitted from the atoms immediately after incident photons are absorbed | A photon may be illuminated at any given time, and its lifetime may very from process to process |
Applications | Mineralogy, fluorescent labeling, use in dyes | Emissive displays, fluorescence lamps, LEDs |