logo
AAT Bioquest

What are the differences between Isoschizomers and Neoschizomers?

Posted April 4, 2024


Answer

Isoschizomers

  • Isoschizomers are restriction enzymes that have the same recognition sequence and cleave the DNA at the same positions. 
  • They have the same specificities and their formation is interchangeable
  • They generate the same types of ends
  • Examples include: Hpall and MSPI, Agel and BshT1, Kpn1 and Acc651

Neoschizomers

  • Neoschizomers are restriction enzymes that have the same recognition sequence but cleave DNA at different positions
  • They have different specificities and their formation is rigid and not interchangeable
  • They generate different types of ends
  • Examples include: Maell and Tail, Smal and Xmal, Apal and Bsp120l
Additional resources

Two different isoschizomers of the type-II restriction endonuclease Taq I (T/CGA) within the same Thermus isolate: Tsp32 I, an enzyme with similar heat stability properties to the prototype enzyme Taq I, and Tsp32 II, a hyperthermostable isoschizomer of Taq I

Enzymes

Restriction Enzymes Cut Sites Reference Table

Amplite® Fluorimetric Coenzyme A Quantitation Kit *Green Fluorescence*