What are the differences between northern and southern blotting?

Question

Accepted Answer

Basis of differentiation

Northern blotting

Southern blotting

Target molecule

RNA

DNA

Probe used

DNA, RNA, and oligodeoxynucleotides

Nucliec acid probe with a homologous sequence sequence with the target molecule

Separation method

Agarose gel electrophoresis

Agarose or denaturing polyacrylamide electrophoresis

Membrane used

DBM or nylon membrane

Nitrocellulose or nylon membrane

Sample preparation

RNA isolation

DNA extraction, enzymatic digestion

Basis of differentiation	Northern blotting	Southern blotting
Target molecule	RNA	DNA
Probe used	DNA, RNA, and oligodeoxynucleotides	Nucliec acid probe with a homologous sequence sequence with the target molecule
Separation method	Agarose gel electrophoresis	Agarose or denaturing polyacrylamide electrophoresis
Membrane used	DBM or nylon membrane	Nitrocellulose or nylon membrane
Sample preparation	RNA isolation	DNA extraction, enzymatic digestion