Basis of differentiation

Northern blotting

Southern blotting

Target molecule

RNA

DNA

Probe used

DNA, RNA, and oligodeoxynucleotides

Nucliec acid probe with a homologous sequence sequence with the target molecule

Separation method

Agarose gel electrophoresis

Agarose or denaturing polyacrylamide electrophoresis 

Membrane used

DBM or nylon membrane

Nitrocellulose or nylon membrane

Sample preparation

RNA isolation

DNA extraction, enzymatic digestion