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AAT Bioquest

What are the differences between one-way and two-way ANOVA?

Posted May 20, 2022


Answer
Basis of DiefferentiationOne-way ANOVATwo-way ANOVA
DefinitionA statistical test used to determine how one factor affects a response variableA statistical test used to determine how two factors affect a response variable, and whether or not there is an interaction effect between the two factors on the response variable
Number of independent variables or factorsInvolves only one independent variable or factorInvolves two independent variables or factors
What the test comparesCompares three or more levels (conditions) of one independent variable or factor Compares the effect of multiple levels of two independent variables or factors 
Number of observations requiredDo not need to be the same in each groupNeed to be equal in each group
Compliance with principles of design of experimentsNeed to comply with only two principles of design of experiments - replication and randomizationNeed to comply with all three principles of design of experiments -replication, randomization, and local control
PurposeTo determine whether the data collected for one dependent variable are close to the common meanTo determine whether the data collected for two dependent variables converge on a common mean derived from two categories
When the test is usedUsed when there is only one independent variable with multiple levelsUsed when there are two independent variables with multiple levels
ExampleTesting the relationship between watering frequency and plant growthTesting the relationship between watering frequency and sunlight exposure on plant growth
Additional resources

Two-way ANOVA for scalar trajectories, with experimental evidence of non-phasic interactions

ANOVA Calculator