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AAT Bioquest

What are the differences between PBS and HBSS?

Posted September 28, 2022


Answer

The differences between PBS and HBSS are listed in the table below. 

PBSHBSS
PBS is a buffered solutionHBSS is a balanced form solution
Is mainly found in liquid formCan be found in liquid or powder form
Is used in biochemistry and molecular biology experiments specificallyIs used widely in biological research in general
Its ingredients are NaCl, KCL, Na2HPO4,  and KH2PO4Its ingredients are inorganic salts, glucose, phenol red, and sodium bicarbonate  
Its storage temperature is between 15-30 degrees CelsiusIts storage temperature is between 4-8 degrees Celsius
Its applications are washing cells, diluting cells, preparing reagents, and transporting tissue It is used for mammalian cell culture and transporting media  
Additional resources

The use of phosphate buffered saline for the recovery of cells and spermatozoa from swabs

Buffer Preparations and Recipes