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What are the differences between restriction enzyme type 1, 2 and 3?"
Posted August 25, 2022
Answer
| Restriction enzyme type 1 | Restriction enzyme type 2 | Restriction enzyme type 3 |
| Type 1 restriction enzymes recognize certain DNA sequences and make cuts at random sites as far as 1000 base pairs away from the recognition site | Type 2 restriction enzymes recognize and cut certain DNA sequences that are all within the recognition site | Recognition enzyme type 3 recognizes specific sequences and cut at a different location that is approximately within 25 base pairs of the recognition site |
| Is a multimeter with 3 subunits | Is a homodimer | Is a heterodimer |
| Restriction and methylation are exclusive reactions | Restriction and methylation are separate reactions | Restriction and methylation reactions happen simultaneously |
| Cleavage happens prior to DNA translocation | No translocation occurs | No translocation occurs |
| Has both restriction and methylase mechanisms | Has separate restriction and methylase activities | Has both restriction and methylase activities |
| Has a bipartite asymmetric restriction recognition site | Has a 4-6 base pair palindromic sequence | Has a 5-7 base pair asymmetric restriction recognition site |
| ATP, MG2+ and S-adenosyl is required for restriction | MG2+ is required for restriction | ATP and Mg2+ is required for restriction |
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