Basis of differentiation | Simple staining | Differential staining |
Definition | Simple staining involves directly staining the microbial cell with only one type of stain | Differential staining uses more than one type of stain |
Colors | It stains a microbial cell only one color | It stains a microbial cell two or more different colors |
Significance | It helps to observe and identify the shape, size, structure, and arrangement of bacterial cells | It helps to differentiate between distinct types of bacterial species |
Example | Methylene blue staining | Gram’s staining, acid fast staining |