Name: MycoLight™ Rapid Fluorescence Gram-Positive Bacteria Staining Kit
Brand: AAT Bioquest
SKU: 22415
Price: 348 USD
Availability: InStock

MycoLight™ Rapid Fluorescence Gram-Positive Bacteria Staining Kit

AAT Bioquest's MycoLight™ Rapid Fluorescence Gram-Positive Bacteria Staining Kit provides a novel one-step fluorescence assay for the determination of gram sign in living bacteria. The gram stain is an important and widely used method for the taxonomic classification of bacteria in clinical and research settings. The original method involves quite a few steps like heat fixation, two-steps staining protocol, alcohol extraction and counterstaining. These steps can create inconsistent staining. AAT Bioquest's one-step kit overcomes the existing problems by eliminating the labor-intensive steps. The kit uses a fluorescently labeled Concanavalin A (ConA), which is a lectin that selectively binds to N-acetyl glucosamine exposed on the surface of gram-positive bacteria. When gram-negative and gram-positive bacteria are stained with the fluorescently labeled ConA conjugate, only gram-positive bacteria fluoresce red. Stained bacteria can be monitored fluorimeterically. Our kit is robust and convenient since the fluorescently labeled ConA conjugate used in our kit demonstrates higher brightness and photo stability over other existing dyes.

<em>Bacillus subtilis (Gram-positive) </em>(A) and <em>Escherichia coli</em> <em>(Gram-negative)</em> (B) was stained with MycoLight™ Rapid Fluorescence Gram-Positive Bacteria Staining Kit.

Protocol

SDS

COA

Catalog	Size	Price	Quantity
22415	100 Tests	Price

Citations

View all 88 citations: Citation Explorer

Vine-inspired zinc-ion modified black phosphorus coating accelerates bone tissue infiltration of 3D printed scaffolds

Authors:

Li, Dan and Dai, Danni and Wang, Jianrong and Wang, Yan and Tian, Yujia and Zhang, Chao

Journal:

Theranostics (2025): 5073

Fish scale-inspired biomimetic nanocoatings on magnesium implants for vascularized bone regeneration in infected bone defects

Authors:

Li, Dan and Dai, Danni and Wang, Jianrong and Ai, Zhen and Zhang, Chao

Journal:

Journal of Magnesium and Alloys (2024)

Staphylococcus pseudintermedius induces pyroptosis of canine corneal epithelial cells by activating the ROS--NLRP3 signaling pathway

Authors:

Wang, Zhihao and Guo, Long and Yuan, Changning and Zhu, Chengcheng and Li, Jun and Zhong, Haoran and Mao, Peng and Li, Jianji and Cui, Luying and Dong, Junsheng and others,

Journal:

Virulence (2024): 2333271

Bacteriocins of Gram-positive bacteria having activity spectra extending beyond closely-related species

Authors:

Todorov, S. D., de Melo Franco, B. D. G., Tagg, J. R.

Journal:

Benef Microbes (2019): 1-14

Coriander essential oil and linalool - interactions with antibiotics against Gram-positive and Gram-negative bacteria

Authors:

Aelenei, P., Rimbu, C. M., Guguianu, E., Dimitriu, G., Aprotosoaie, A. C., Brebu, M., Horhogea, C. E., Miron, A.

Journal:

Lett Appl Microbiol (2019): 156-164

Storage, safety and handling

H-phrase	H303, H313, H333
Hazard symbol	XN
Intended use	Research Use Only (RUO)
R-phrase	R20, R21, R22
UNSPSC	12352200

Instrument settings

Fluorescence microscope
Excitation	650 nm
Emission	669 nm
Recommended plate	Black wall/clear bottom
Instrument specification(s)	Cy5 filter

Telephone
Fax
Email	sales@aatbio.com
International	See distributors
Bulk request	Inquire
Custom size	Inquire
Technical Support	Contact us
Request quotation	Request
Purchase order	Send to sales@aatbio.com
Shipping	Standard overnight for United States, inquire for international

Page updated on September 23, 2025

Component A: IF647-ConA	2 vials
Component B: Assay Buffer	1 bottle (25 mL)