What are the disadvantages of long-read sequencing?

One disadvantage is that long-read sequencing technologies typically have higher error rates compared to short-read sequencing methods, particularly in the base-calling accuracy of individual reads. Long-read sequencing platforms typically have lower throughput compared to short-read sequencing instruments, resulting in fewer reads generated per sequencing run. These technologies are often more expensive compared to short-read sequencing methods, both in terms of instrument costs and per-base sequencing costs. Lastly, they often require longer sequencing times compared to short-read sequencing methods.