AAT Bioquest

What are the disadvantages of Nanodrop light spectrophotometer?

Posted September 26, 2022


There are various disadvantages that arise from using the nanodrop light spectrophotometer. One disadvantage is that the machine is susceptible to contamination, as the sample readings are susceptible to foreign matter. For example, since DNA and RNA have absorption at 260 nm, both can overestimate the concentration readings. Another disadvantage is that cleaning is required. If the machine is not properly cleaned, the data may be affected. The next disadvantage is that there is a lack of sensitivity. It is recommended to not measure samples with concentration below 10 ng/mL due to possible inaccurate readings. Lastly, Nanodrop cannot determine from the sample whether it is DNA, RNA, or protein.          

Additional resources

Spexyte™ Micro pH Probe *Optimized for Nanodrop*

Quantification of DNA through the NanoDrop Spectrophotometer: Methodological Validation Using Standard Reference Material and Sprague Dawley Rat and Human DNA