What are the factors that affect the actual yield of the sorted cell samples?
Posted May 24, 2023
Main factors that affect the actual yield of the sorted cell samples include:
- Electronic aborts: This occurs when cells are too close to each other in the laser beam, leading to the removal and non-processing of multiple cells with each electronic abort.
- Sort conflicts: Sort conflicts may arise when a target and non-target cell are present within the same or overlapping sort envelopes, resulting in the non-sorting of both cell types.
- Increasing the sample rate: Increasing the number of cells analyzed per second and the presence of cell aggregates leads to more losses and lower yield.
- Cell death: Loss of target cells may occur due to cell death before or after sorting, or when cells adhere to tube walls.