Main factors that affect the actual yield of the sorted cell samples include: 

• Electronic aborts: This occurs when cells are too close to each other in the laser beam, leading to the removal and non-processing of multiple cells with each electronic abort. 
• Sort conflicts: Sort conflicts may arise when a target and non-target cell are present within the same or overlapping sort envelopes, resulting in the non-sorting of both cell types. 
• Increasing the sample rate: Increasing the number of cells analyzed per second and the presence of cell aggregates leads to more losses and lower yield.  
• Cell death: Loss of target cells may occur due to cell death before or after sorting, or when cells adhere to tube walls.