What effect will the cell sorting process have on my cells' viability?

The effect of the cell sorting process on your cells’ viability will depend on a number of factors.

• Effect of cell type: Some types of cells are more fragile and unstable than others. Cells that feature structures on the outer surface of the cell membrane are more vulnerable to damage during the sorting process. 
• Effect of cell condition prior to sorting: The effects of sorting may be more pronounced in cells that have been activated or treated with drugs. Additionally, when subjected to higher pressures, apoptotic cells are likely to be selected against. 
• Effect of pressure at which cells are sorted: You have to use a higher pressure if you want to speed up the sorting process. However, this may not necessarily be the best option as some cells are more susceptible to damage when subjected to high pressure. 
• Effect of buffer used for sorting: It’s best to avoid significant mixing of buffers as most cells are sensitive to changes in buffer conditions. Mixing buffers, particularly carbonate buffers with phosphate buffers (sorting fluid), can lead to the formation of precipitates on the cell membranes, which can be detrimental to cell viability.