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What are the factors that might cause the overestimation of live cells in PMA-treated samples?

Posted July 17, 2024


Chemical Reagents
Cell Viability Assays
Cellular Structures and Organelles
Live or Dead
Physiological Probes
Plasma Membrane
Answer

The two main factors that may potentially cause the overestimation of live cells in PMA-treated samples are:

  1. Using smaller amplicon lengths 
  2. Live:dead cell ratios higher than 1:999
Additional resources

Limitations of Using Propidium Monoazide with qPCR to Discriminate between Live and Dead Legionella in Biofilm Samples

Cell Viability Assays

Calcein, AM *UltraPure grade* *CAS 148504-34-1*

Related questions
How does viability PCR (vPCR) work?
What are the commonly used viability PCR reagents?
What is Propidium Monoazide (PMA)?
What is the most important criteria for distinguishing between viable and irreversibly damaged cells?
What is viability PCR (vPCR)?