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AAT Bioquest

What are the factors that might cause the overestimation of live cells in PMA-treated samples?

Posted July 17, 2024


Answer

The two main factors that may potentially cause the overestimation of live cells in PMA-treated samples are:

  1. Using smaller amplicon lengths 
  2. Live:dead cell ratios higher than 1:999
Additional resources

Limitations of Using Propidium Monoazide with qPCR to Discriminate between Live and Dead Legionella in Biofilm Samples

Cell Viability Assays

Calcein, AM *UltraPure grade* *CAS 148504-34-1*