What are the factors that might cause the overestimation of live cells in PMA-treated samples? | AAT Bioquest

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What are the factors that might cause the overestimation of live cells in PMA-treated samples?

Posted July 17, 2024

Chemical Reagents Cell Viability Assays Cellular Structures and Organelles Live or Dead Physiological Probes Plasma Membrane

Answer

The two main factors that may potentially cause the overestimation of live cells in PMA-treated samples are:

Using smaller amplicon lengths
Live:dead cell ratios higher than 1:999

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