What is viability PCR (vPCR)?

Posted July 17, 2024

Chemical Reagents Cell Viability Assays Cellular Structures and Organelles Physiological Probes Plasma Membrane Polymerase Chain Reaction (PCR)

Answer

Viability PCR (vPCR) is an advanced version of PCR that allows for the detection of only live cells by neutralizing the DNA of dead cells through a pre-treatment process using specific intercalating photo-reactive reagents.

Additional resources

Use of viability PCR for detection of live Chlamydia trachomatis in clinical specimens Cell Viability Assays Calcein, AM *UltraPure grade* *CAS 148504-34-1* Polymerase Chain Reaction (PCR)

What is Propidium Monoazide (PMA)?What are the commonly used viability PCR reagents?How does viability PCR (vPCR) work?What are the factors that might cause the overestimation of live cells in PMA-treated samples?What are the applications of viability PCR (vPCr)?

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