What is Propidium Monoazide (PMA)? | AAT Bioquest

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What is Propidium Monoazide (PMA)?

Posted July 17, 2024

Chemical Reagents Cell Viability Assays Cellular Structures and Organelles DNA and RNA Quantitation Physiological Probes Plasma Membrane Polymerase Chain Reaction (PCR)

Answer

PMA or Propidium Monoazide is a highly photoreactive, membrane-impermeable, DNA-intercalating dye that belongs to the phenanthridine family. It is widely used in viability PCR (vPCR) assays to discriminate between viable and nonviable microbial cells.

Additional resources

Applicability of propidium monoazide (PMA) for discrimination between living and dead phytoplankton cells Polymerase Chain Reaction (PCR) Cell Viability Assays Calcein, AM *UltraPure grade* *CAS 148504-34-1*

What is viability PCR (vPCR)?What are the commonly used viability PCR reagents?How does viability PCR (vPCR) work?What are the factors that might cause the overestimation of live cells in PMA-treated samples?What are the applications of viability PCR (vPCr)?