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What are the limitations of cross-linking immobilization?
Posted February 22, 2024
Antibodies and Proteomics BioconjugationCellular ProcessesCellular Structures and OrganellesEnzymes
Answer
Cross-linking is an enzyme immobilization technique in which enzymes are cross-linked with each other without any support material, so the enzymes act as their carrier. 
Disadvantages of Cross Linking 
  • Requires enzymes to be in crystallized form, which requires highly pure enzymes, making this a very expensive technique
  • Inhibits enzyme activity 
  • Enzymes are susceptible to denaturation by the polyfunctional chemicals used in this method
Additional resources
(Magnetic) Cross-Linked Enzyme Aggregates of Cellulase from T. reesei: A Stable and Efficient Biocatalyst EnzymesAmplite® Fluorimetric Coenzyme A Quantitation Kit *Green Fluorescence*

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