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AAT Bioquest

What are the limitations of Restriction Fragment Length Polymorphism (RFLP)?

Posted June 25, 2024


Answer

One con is that the RFLP procedure requires various steps and takes weeks to achieve results when not combined with PCR. RFLP analysis also necessitates a larger quantity of extracted DNA compared to other methods like RAPD. Additionally, automation is not possible and only a few loci are detected per assay. 

Additional resources

Restriction Fragment Length Polymorphism

DNA Fragmentation

Gelite™ Green Nucleic Acid Gel Staining Kit