Basis of differentiation | RAPD | RFLP |
Definition | RAPD is a molecular marker based on random primers and PCR | RFLP is a genetic marker that relies on the generation of DNA fragments of varying lengths after DNA samples are treated with specific restriction enzymes |
Time | Is time efficient | Is comparatively less time efficient |
Required sample | RAPD analysis requires only a small amount of DNA sample | RFLP analysis necessitates a larger quantity of extracted DNA |
Primer | Random primers are used in DNA amplification and these primers can be utilized across different species | Utilizes probes that are specific to particular species for hybridization |
Detection of allelic variations | RAPD cannot detect allelic variations | Can detect allelic variations |
Requirement for sequence knowledge | Does not require sequence knowledge beforehand | Requires prior sequence knowledge for probe design |
Reliability | Less reliable | More reliable |
Reproducibility | Exhibits low reproducibility | Exhibits higher reproducibility |