What are the differences between Random Amplified Polymorphic DNA (RAPD) and RFLP?

Basis of differentiation

RAPD

RFLP

Definition

RAPD is a molecular marker based on random primers and PCR

RFLP is a genetic marker that relies on the generation of DNA fragments of varying lengths after DNA samples are treated with specific restriction enzymes

Time 

Is time efficient

Is comparatively less time efficient

Required sample

RAPD analysis requires only a small amount of DNA sample

RFLP analysis necessitates a larger quantity of extracted DNA

Primer

Random primers are used in DNA amplification and these primers can be utilized across different species 

Utilizes probes that are specific to particular species for hybridization 

Detection of allelic variations

RAPD cannot detect allelic variations

Can detect allelic variations 

Requirement for sequence knowledge

Does not require sequence knowledge beforehand

Requires prior sequence knowledge for probe design

Reliability

Less reliable

More reliable

Reproducibility

Exhibits low reproducibility 

Exhibits higher reproducibility