What are the main differences between a primary antibody and a secondary antibody?
Posted June 27, 2022
|Basis of Differentiation||Primary Antibody||Secondary Antibody|
|Definition||Is an immunoglobulin that binds directly to a specific protein or other target biomolecule||Is designed to bind to the primary antibody, which is directly bound to the target antigen|
|Use in immunoassays||Is necessary to bind to the target antigen||Is not always necessary|
|Interation with antigen||Interacts directly with the antigen||Interacts indirectly with the antigen by binding with the primary antibody|
|Source||Host species of the primary antibody must be different from the species of the sample||Host species of the secondary antibody must be different from the host species of the primary antibody|
|Function||Used as biomarkers for detecting diseases such as Alzheimer’s disease, cancer, Parkinson’s disease and diabetes.||Used in immunolabeling|