AAT Bioquest

What are the major steps involved in RNA processing?

Posted October 22, 2022


There are three main steps for RNA processing.

  • The first step of RNA processing involves capping at the 5’ end. A methylated-guanosine connects to the phosphates at the 5’ end of the mRNA.
  • Next, a polyA tail is added to the 3’ end. The 3’ end of a eukaryotic mRNA is shortened, and the enzyme Poly(A) polymerase adds a tail of about 200 ‘A’ nucleotides to the 3’ end.
  • Lastly, introns are removed from the pre-mRNA through splicing. 
Additional resources

RNA Processing

Cell Navigator® Live Cell RNA Imaging Kit *Green Fluorescence*