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What are the pros of membrane asymmetry cell viability assays?
Posted June 21, 2023
Annexin-V StainingApoptosis and NecrosisCell Viability AssaysCellular ProcessesChemical ReagentsClassic Dyes
Answer

Pros of membrane asymmetry cell viability assays: 

  • F2N12S (4′-N,N-diethylamino-6-(N-dodecyl-N-methyl-N-(3-sulfopropyl))ammoniomethyl-3-hydroxyflavone) staining is very quick (5 minutes).
  • F2N12S probe is a tiny molecule and is not sensitive to proteases.
  • Fixation is possible with annexin V staining.
  • Both dyes – F2N12S and annexin V – can be combined with other markers for intracellular functional analysis or for cell surface phenotyping.
  • Membrane permeabilization is not needed for either of the dyes.
Additional resources
Erythrocyte membrane defects and asymmetry in paroxysmal nocturnal hemoglobinuria and myelodysplastic syndromeCell Viability Assays Annexin V, FITC Labeled

How do you detect apoptosis?What are the cons of membrane asymmetry cell viability assays?Can apoptosis be detected by cell cycle studies?Why are Annexin V conjugates and 7-AAD used together?Are Annexin V-FITC+/PI+ cells classified as late-apoptotic cells or necrotic cells?