AAT Bioquest

What are the pros of membrane asymmetry cell viability assays?

Posted June 21, 2023


Pros of membrane asymmetry cell viability assays

  • F2N12S (4′-N,N-diethylamino-6-(N-dodecyl-N-methyl-N-(3-sulfopropyl))ammoniomethyl-3-hydroxyflavone) staining is very quick (5 minutes).
  • F2N12S probe is a tiny molecule and is not sensitive to proteases.
  • Fixation is possible with annexin V staining.
  • Both dyes – F2N12S and annexin V – can be combined with other markers for intracellular functional analysis or for cell surface phenotyping.
  • Membrane permeabilization is not needed for either of the dyes.
Additional resources

Erythrocyte membrane defects and asymmetry in paroxysmal nocturnal hemoglobinuria and myelodysplastic syndrome

Cell Viability Assays

Annexin V, FITC Labeled