What are the pros of RNA sequencing?

One advantage is that it requires only a small amount of starting material because there are no cloning steps. Typically, only 100 nanograms to 1 microgram of total RNA is sufficient for high quality RNA. For low-quality or degraded RNA, more starting material may be required. Another advantage is that prior sequencing information is not required. This makes RNA-Seq particularly useful for non-model organisms with genomic sequences that are still unknown. RNA-Seq can display the exact location of transcription boundaries, to a single-base resolution. RNA-seq also has very low, if any, background signal because DNA sequences can be unequivocally mapped to unique regions of the genome. It can also display sequence variations in the transcribed regions. Another advantage is that they have a large dynamic range of expression levels over which transcripts can be detected. Additionally, RNA-Seq is highly accurate for quantifying expression levels, as determined using qPCR. They also display high levels of reproducibility.