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What are the steps of the synthesis of aminoacyl-tRNAs
Posted July 10, 2023
ATPCell SignalingCellular ProcessesDNA and RNAEnzymesProteinamino acid sequence
Answer
Aminoacyl-tRNAs are synthesized in two steps: 
  1. The first step involves ATP (Adenosine Triphosphate) - dependent adenylation of the amino acid, resulting in the formation of aminoacyl-AMP (Adenosine Monophosphate).
The net reaction in this step is: ATP + Amino acid = Aminoacyl-AMP + PPi(Pyrophosphate)
  1. In the second step, the amino acid residue is transferred to the tRNA (Transfer RNA).
The net reaction in this step is: ATP + Amino Acid + tRNA = Aminoacyl-tRNA + AMP + PPi
Additional resources
Aminoacyl-tRNA synthetasesCell SignalingHelixyte™ Green *10,000X Aqueous PCR Solution*6-ROX glycine *25 uM fluorescence reference solution for PCR reactions*

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