AAT Bioquest

What is the difference between Sanger sequencing and PCR?

Posted July 13, 2022


Sanger Sequencing and PCR or Polymerase Chain Reaction are both DNA sequencing methods. They use similar starting materials and can be used in conjunction with each other, but neither one can replace the other. The basic difference between the two is that Sanger sequencing is used to generate every possible length of DNA up to the full length of the target DNA while PCR is used to duplicate the entire DNA sequence. 

Additional resources

Polymerase Chain Reaction (PCR)

PCR and DNA sequencing

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