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AAT Bioquest

What is the purpose of a mounting medium in biology?

Posted April 6, 2023


Answer

Using a mounting medium is important when you want to observe a specimen under a microscope. The mounting medium serves several important purposes: 

  • Helps hold the sample in place while you are imaging. 
  • Mechanically stabilizes and supports the sample so delicate structures don’t break off. 
  • Prevents the sample from drying out. 
  • Prevents moisture and bacteria from coming in contact with the sample, causing it to decompose. 
  • Reduces photobleaching 
  • Improves image quality when its refractive index (RI) closely matches the RI for the objective you will use
  • Preserves sample for long-term storage
Additional resources

High-throughput live-imaging of embryos in microwell arrays using a modular specimen mounting system

FluoroQuest™ PLUS Antifade Mounting Medium