AAT Bioquest

What is whole genome sequencing?

Posted June 23, 2022


Whole genomes sequencing (WGS) is a laboratory technique used to view and analyze the entire genome. This process enables researchers to determine almost all of the nucleotides of an individual’s complete DNA sequence, including the coding and noncoding sequences. WGS is ideal for discovery applications such as the identification of causative variants and novel genome assembly.

Whole genome sequencing offers several advantages over more focused approaches such as exome sequencing or targeted resequencing. It is a faster and more cost-effective way to obtain high-level information about the target bacteria with just one test. It enables the detection of single nucleotide variants, large structural variants, insertions/deletions and copy number changes and delivers large volumes of data in a relatively short amount of time. Recent technological innovations have significantly improved the functionality, efficiency, and cost-effectiveness of whole genome sequencing. 

Additional resources

Whole genome sequencing

MagaDye™ 4 Color Sanger Sequencing Terminator Kit

DNA and RNA Quantitation