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How many primers are needed for Sanger sequencing?
Posted July 11, 2022
DNA and RNA QuantitationPhysiological ProbesCoronavirus (SARS-CoV-2)Nucleus
Answer

Only one primer is needed for Sanger sequencing. This is different from PCR amplification which requires two primers from opposite strands.  

Additional resources
Polymerase Chain Reaction (PCR)MagaDye™ 4 Color Sanger Sequencing Terminator KitNoncontinuously binding loop-out primers for avoiding problematic DNA sequences in PCR and sanger sequencing

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