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How many primers are needed for Sanger sequencing?

Posted July 11, 2022


DNA and RNA Quantitation
Physiological Probes
Coronavirus (SARS-CoV-2)
Nucleus
Answer

Only one primer is needed for Sanger sequencing. This is different from PCR amplification which requires two primers from opposite strands.  

Additional resources

Polymerase Chain Reaction (PCR)

MagaDye™ 4 Color Sanger Sequencing Terminator Kit

Noncontinuously binding loop-out primers for avoiding problematic DNA sequences in PCR and sanger sequencing

Related questions
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Location: Home / Frequently Asked Questions (FAQ) / How many primers are needed for Sanger sequencing?
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