AAT Bioquest

What kind of washing buffer should I use for immunoprecipitation protocols?

Posted November 8, 2022


 When choosing a washing buffer for immunoprecipitation protocols, it is important to create conditions that support the desired protein interactions while preventing non-specific protein binding. In most cases PBS or TBS provide the ideal starting point.

Additional resources

Affinity Pull-Down of Proteins Using Anti-FLAG M2 Agarose Beads

Buffer Preparations and Recipes