logo
Products
Technologies
Applications
Services
Resources
Selection Guides
About
What's the significance of oxidase tests?
Posted May 9, 2024

Answer

The significance of oxidase tests is that they help to identify microorganisms that contain the enzyme cytochrome oxidase. The oxidase test involves adding a colorless reagent tetra-methyl- p-phenylenediamine dihydrochloride (TMPD) to a sample. If the cytochrome c oxidase enzyme is present, TMPD gets oxidized and changes from colorless to a purple or dark blue compound. If cytochrome c is absent, TMPD remains reduced and colorless.