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Amplite® Fluorimetric Monoamine Oxidase Assay Kit
Red Fluorescence
Monoamine oxidases (MAO) are a family of flavin-containing amine oxidoreductases that catalyze the oxidation of monoamines. They are found bound to the outer membrane of mitochondria in numerous tissues including liver, intestinal mucosa, and nerves. In humans there are two types of MAO: MAO-A and MAO-B. MAO-A is particularly important in the metabolism of monoamines ingested in food. MAOs play a major role in the inactivation of neurotransmitters. MAO dysfunction has been associated with depression, schizophrenia, substance abuse, attention deficit disorder, migraines, and irregular sexual maturation. The Amplite® Monoamine Oxidase Assay Kit provides a quick and sensitive method for the measurement of monoamine oxidase and semicarbazide-sensitive amine oxidase (SSAO) activities in blood samples and other biological samples. The kit uses our Amplite® Red substrate which enables a dual recordable mode. The signal can be easily read by either a fluorescence microplate reader or an absorbance microplate reader. With the Amplite® Monoamine Oxidase Assay Kit, we have detected as little as 1 mU/mL SSAO in a 100 µL reaction volume. It can be performed in a convenient 96-well or 384-well microtiter-plate format and easily adapted to automation.
PAO dose response was obtained with Amplite® Fluorimetric Monoamine Oxidase Assay Kit in a 96-well solid black plate using a Gemini fluorescence microplate reader (Molecular Devices).
PAO dose response was obtained with Amplite® Fluorimetric Monoamine Oxidase Assay Kit in a 96-well solid black plate using a Gemini fluorescence microplate reader (Molecular Devices).
CatalogSize
Price
Quantity
11303200 Tests
Price
 
Spectral properties

Excitation (nm)571
Emission (nm)584
Storage, safety and handling

H-phraseH303, H313, H333
Hazard symbolXN
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R22
UNSPSC12171501
Instrument settings

Fluorescence microplate reader
Excitation540nm
Emission590nm
Cutoff570nm
Recommended plateSolid black
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Page updated on October 9, 2025