Name: Amplite® Fluorimetric Monoamine Oxidase Assay Kit *Red Fluorescence*
Brand: AAT Bioquest
SKU: 11303
Price: 237 USD
Availability: InStock

Amplite® Fluorimetric Monoamine Oxidase Assay Kit

Red Fluorescence

Amplite® Fluorimetric Monoamine Oxidase Assay Kit *Red Fluorescence* is a fluorimetric assay kit for measurement of monoamine oxidase and semicarbazide-sensitive amine oxidase (SSAO) activities in blood and other biological samples.

Detection Principle: Measures amine oxidase activity using an Amplite® Red/HRP-coupled fluorescent reaction.
Sensitivity: Detects SSAO activity down to 1 mU/mL in a 100 µL reaction.
Workflow: Compatible with 96- or 384-well microplates and can be adapted to automation.
Instrument Compatibility: Supports fluorescence detection at Ex/Em 540/590 nm.

PAO dose response was obtained with Amplite® Fluorimetric Monoamine Oxidase Assay Kit in a 96-well solid black plate using a Gemini fluorescence microplate reader (Molecular Devices).

Protocol

SDS

Catalog	Size	Price	Quantity
11303	200 Tests	Price

Overview

Monoamine oxidases (MAO) are a family of flavin-containing amine oxidoreductases that catalyze the oxidation of monoamines. They are found bound to the outer membrane of mitochondria in numerous tissues including liver, intestinal mucosa, and nerves. In humans there are two types of MAO: MAO-A and MAO-B. MAO-A is particularly important in the metabolism of monoamines ingested in food. MAOs play a major role in the inactivation of neurotransmitters. MAO dysfunction has been associated with depression, schizophrenia, substance abuse, attention deficit disorder, migraines, and irregular sexual maturation. The Amplite® Monoamine Oxidase Assay Kit provides a quick and sensitive method for the measurement of monoamine oxidase and semicarbazide-sensitive amine oxidase (SSAO) activities in blood samples and other biological samples. The kit uses our Amplite® Red substrate which enables a dual recordable mode. The signal can be easily read by either a fluorescence microplate reader or an absorbance microplate reader. With the Amplite® Monoamine Oxidase Assay Kit, we have detected as little as 1 mU/mL SSAO in a 100 µL reaction volume. It can be performed in a convenient 96-well or 384-well microtiter-plate format and easily adapted to automation.

References

View all 58 references: Citation Explorer

An association study of monoamine oxidase A (MAOA) gene polymorphism in methamphetamine psychosis

Authors:

Nakamura K, Sekine Y, Takei N, Iwata Y, Suzuki K, Anitha A, Inada T, Harano M, Komiyama T, Yamada M, Iwata N, Iyo M, Sora I, Ozaki N, Ujike H, Mori N.

Journal:

Neurosci Lett (2009): 120

Platelet serotonin concentration and monoamine oxidase type B activity in female patients in early, middle and late phase of Alzheimer's disease

Authors:

Muck-Seler D, Presecki P, Mimica N, Mustapic M, Pivac N, Babic A, Nedic G, Folnegovic-Smalc V.

Journal:

Prog Neuropsychopharmacol Biol Psychiatry (2009): 1226

Altered platelet monoamine oxidase-B activity in idiopathic Parkinson's disease

Authors:

Husain M, Shukla R, Dikshit M, Maheshwari PK, Nag D, Srimal RC, Seth PK, Khanna VK.

Journal:

Neurochem Res (2009): 1427

Chronic treatment with monoamine oxidase-B inhibitors decreases cocaine reward in mice

Authors:

Ho MC, Cherng CG, Tsai YP, Chiang CY, Chuang JY, Kao SF, Yu L.

Journal:

Psychopharmacology (Berl) (2009): 141

Serine 209 resides within a putative p38(MAPK) consensus motif and regulates monoamine oxidase-A activity

Authors:

Cao X, Rui L, Pennington PR, Chlan-Fourney J, Jiang Z, Wei Z, Li XM, Edmondson DE, Mousseau DD.

Journal:

J Neurochem (2009): 101

Spectral properties

Excitation (nm)	571
Emission (nm)	584

Usage and storage

Intended use

Research Use Only (RUO)

Instrument settings

Fluorescence microplate reader
Excitation	540nm
Emission	590nm
Cutoff	570nm
Recommended plate	Solid black

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Page updated on October 8, 2026

Component A: Amplite® Red (light sensitive)	1 vial
Component B: Assay Buffer	1 bottle (20 mL)
Component C: Horseradish Peroxidase (lyophilized)	1 vial
Component D: MAO Substrate	1 vial (50 µL)
Component E: Plasma Amine Oxidase Standard (lyophilized)	1 vial (2.5 Units)
Component F: DMSO	1 vial (200 µL)