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Amplite® Fluorimetric Lysyl Oxidase Assay Kit
Red Fluorescence
Lysyl oxidase (LOX) is an extracellular enzyme that catalyzes formation of aldehydes from lysine residues in collagen and elastin precursors. These aldehydes are highly reactive, and undergo spontaneous chemical reactions with other lysyl oxidase-derived aldehyde residues, or with unmodified lysine residues. This results in cross-linking collagen and elastin which is essential for stabilization of collagen fibrils and for the integrity and elasticity of mature elastin. Lysyl oxidase has been identified as a possible tumor suppressor. Lysyl oxidase activity in biological samples is traditionally and most reliably assessed by tritium release end-point assays using radiolabeled collagen or elastin substrates involving laborious vacuum distillation of the released tritiated water. This kit offers a sensitive fluorescent assay to measure LOX activity using our proprietary LOX substrate that releases hydrogen peroxide upon LOX oxidation. The amount of hydrogen peroxide released by the LOX oxidation is detected using our Amplite® HRP substrate in the HRP-coupled reactions. This method allows the detection of sub ng/mL lysyl oxidase and is much more sensitive than the currently available fluorimetric assay for this enzyme activity. This method eliminates the interference that occurs in some biological samples and can be readily used to detect lysyl oxidase activity in cell culture experiments. Please note that the kit does not include the lysyl oxidase enzyme.
Lysyl oxidase dose response was measured with Amplite® Fluorimetric Lysyl Oxidase Assay Kit on a solid black 96-well plate using a Gemini fluorescence microplate reader (Molecular Devices).
Lysyl oxidase dose response was measured with Amplite® Fluorimetric Lysyl Oxidase Assay Kit on a solid black 96-well plate using a Gemini fluorescence microplate reader (Molecular Devices).
CatalogSize
Price
Quantity
15255500 Tests
Price
 
Spectral properties

Excitation (nm)571
Emission (nm)584
Storage, safety and handling

H-phraseH303, H313, H333
Hazard symbolXN
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R22
UNSPSC12352200
Instrument settings

Fluorescence microplate reader
Excitation540 nm
Emission590 nm
Cutoff570 nm
Recommended plateSolid black
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Page updated on October 13, 2025