Name: Amplite® Fluorimetric Lysyl Oxidase Assay Kit *Red Fluorescence*
Brand: AAT Bioquest
SKU: 15255
Price: 355 USD
Availability: InStock

Amplite® Fluorimetric Lysyl Oxidase Assay Kit

Red Fluorescence

Detection Principle: Measures H₂O₂ released during LOX-mediated oxidation of a proprietary substrate.
Sensitivity: Detects lysyl oxidase at sub-ng/mL levels. AAT Bioquest
Detection Chemistry: Uses Amplite® HRP substrate in an HRP-coupled reaction.
Workflow: Incubates at 37°C for 10–30 minutes.
Instrument Compatibility: Fluorescence detection at Ex/Em 540/590 nm using solid black microplates.

Lysyl oxidase dose response was measured with Amplite® Fluorimetric Lysyl Oxidase Assay Kit on a solid black 96-well plate using a Gemini fluorescence microplate reader (Molecular Devices).

Protocol

SDS

Catalog	Size	Price	Quantity
15255	500 Tests	Price

Overview

Lysyl oxidase (LOX) is an extracellular enzyme that catalyzes formation of aldehydes from lysine residues in collagen and elastin precursors. These aldehydes are highly reactive, and undergo spontaneous chemical reactions with other lysyl oxidase-derived aldehyde residues, or with unmodified lysine residues. This results in cross-linking collagen and elastin which is essential for stabilization of collagen fibrils and for the integrity and elasticity of mature elastin. Lysyl oxidase has been identified as a possible tumor suppressor. Lysyl oxidase activity in biological samples is traditionally and most reliably assessed by tritium release end-point assays using radiolabeled collagen or elastin substrates involving laborious vacuum distillation of the released tritiated water. This kit offers a sensitive fluorescent assay to measure LOX activity using our proprietary LOX substrate that releases hydrogen peroxide upon LOX oxidation. The amount of hydrogen peroxide released by the LOX oxidation is detected using our Amplite® HRP substrate in the HRP-coupled reactions. This method allows the detection of sub ng/mL lysyl oxidase and is much more sensitive than the currently available fluorimetric assay for this enzyme activity. This method eliminates the interference that occurs in some biological samples and can be readily used to detect lysyl oxidase activity in cell culture experiments. Please note that the kit does not include the lysyl oxidase enzyme.

Citations

View all 61 citations: Citation Explorer

Breaking physical and immunological barriers: a neutrophil-mimicking nanotherapeutics for enhanced pancreatic cancer immunotherapy

Authors:

Wei, Dan and Xi, Zihan and Sun, Jingyi and Cheng, Xiaoyu and Yuan, Xiaoliang and Liu, Kun and Peng, Wei and Wang, Jianlin and Tao, Kaishan and Cao, Tianyu

Journal:

Journal of the National Cancer Center (2026)

Bone marrow fibrocytes and endothelial-to-mesenchymal transition drive pathological ECM remodeling in proliferative diabetic retinopathy

Authors:

Xiao, Ting-ting and Wang, Bin and Qu, Xiao-yong and You, Zhi-peng

Journal:

Molecular and Cellular Biochemistry (2026): 1--13

ATP7A-fibulin-4 complex delivers copper in the Golgi to activate LOX in renal fibrosis

Authors:

Zhou, Wenqian and Zheng, Yan and Liu, Yuqing and Liu, Jing and Liu, Yiguo and Niu, Yangyang and Yu, Ying and Zhang, Xiaoqin and Zhang, Yingying and Yu, Chen

Journal:

JCI insight (2026)

Intestinal Epithelial-like Cells Stimulated by a Functional Food Ingredient Promote Lysyl Oxidase (LOX) Expression in Osteoblast Precursor Cells via BMP-1 Secretion

Authors:

Fujimoto, Naoki and Suzuki, Shotaro and Yano, Tomohiro and Sakata, Shinji and Ito, Yuka and Ishida, Tatsuya

Journal:

International Journal of Molecular Sciences (2026): 3156

Lysyl oxidase promotes seminal vesicle atrophy and fibrosis in type-2 diabetes mellitus through TGF-$\beta$1 signaling

Authors:

Yu, Yi and Liu, Peng-Fei and Zhan, Ming-Wei and Chen, Yao-Qi and Lai, Yu-Qi and Wang, Lei and Wu, Jun-Jie and Yao, Fan and Chen, Xue-Qin and Shang, Xue-Jun and others,

Journal:

Journal of Translational Medicine (2025): 1--15

References

View all 21 references: Citation Explorer

Overexpression of lysyl oxidase to increase matrix crosslinking and improve tissue strength in dermal wound healing

Authors:

Lau YK, Gobin AM, West JL.

Journal:

Ann Biomed Eng (2006): 1239

Cellular fibronectin binds to lysyl oxidase with high affinity and is critical for its proteolytic activation

Authors:

Fogelgren B, Polgar N, Szauter KM, Ujfaludi Z, Laczko R, Fong KS, Csiszar K.

Journal:

J Biol Chem (2005): 24690

The Pro-regions of lysyl oxidase and lysyl oxidase-like 1 are required for deposition onto elastic fibers

Authors:

Thomassin L, Werneck CC, Broekelmann TJ, Gleyzal C, Hornstra IK, Mecham RP, Sommer P.

Journal:

J Biol Chem (2005): 42848

A molecular role for lysyl oxidase-like 2 enzyme in snail regulation and tumor progression

Authors:

Peinado H, Del Carmen Iglesias-de la Cruz M, Olmeda D, Csiszar K, Fong KS, Vega S, Nieto MA, Cano A, Portillo F.

Journal:

Embo J (2005): 3446

Lysyl oxidase is essential for normal development and function of the respiratory system and for the integrity of elastic and collagen fibers in various tissues

Authors:

Maki JM, Sormunen R, Lippo S, Kaarteenaho-Wiik R, Soininen R, Myllyharju J.

Journal:

Am J Pathol (2005): 927

Spectral properties

Excitation (nm)	571
Emission (nm)	584

Usage and storage

Intended use

Research Use Only (RUO)

Instrument settings

Fluorescence microplate reader
Excitation	540 nm
Emission	590 nm
Cutoff	570 nm
Recommended plate	Solid black

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Email	sales@aatbio.com
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Page updated on October 7, 2026

Component A: Amplite® HRP Substrate (light sensitive)	1 vial
Component B: Assay Buffer	1 bottle (50 mL)
Component C: Horseradish Peroxidase	1 vial (50 units)
Component D: DMSO	1 vial (200 µL)