Amplite® Fluorimetric Glutamate Oxidase Assay Kit *Red Fluorescence*

Amplite® Fluorimetric Glutamate Oxidase Assay Kit

Red Fluorescence

Amplite® Fluorimetric Glutamate Oxidase Assay Kit *Red Fluorescence* is a fluorimetric assay kit for quantification of glutamate oxidase activity in solution and cell lysates.

Detection Principle: Measures H₂O₂ generated during L-glutamate oxidation. AAT Bioquest
Sensitivity: Detects glutamate oxidase down to 40 µU/mL.
Detection Chemistry: Uses Amplite® Red with horseradish peroxidase coupling.
Instrument Compatibility: Fluorescence detection at Ex/Em 540/590 nm.

Glutamate oxidase dose response was measured with Amplite® Fluorimetric Glutamate Oxidase Assay Kit in a 96-well solid black plate using a Gemini fluorescence microplate reader (Molecular Devices).

Protocol

SDS

Catalog	Size	Price	Quantity
11302	200 Tests	Price

Overview

Glutamate oxidase belongs to the family of oxidoreductases, specifically those acting on the CH-NH2 group of donors with oxygen as an acceptor. It is an enzyme that specifically catalyzes the oxidative deamination of L-glutamate in the presence of water and oxygen with the formation of o-ketoglutarate, ammonia, and hydrogen peroxide. The Amplite® Fluorimetric Glutamate Oxidase Assay Kit provides a quick and ultrasensitive method for the measurement of glutamate oxidase in solution and in cell lysates. In the assay, L-glutamic acid is oxidized to µ-ketoglutarate, NH3 and H2O2 by glutamate oxidase. L-Alanine and L-glutamate-pyruvate transaminase are included in the reaction, resulting in multiple cycles of the initial reaction, thus significantly amplifying the production of H2O2. The kit uses our Amplite® Red substrate which enables a dual recordable mode. The fluorescent signal can be easily read by either a fluorescence microplate reader or an absorbance microplate reader. With the Amplite® Glutamate Oxidase Assay kit, we have detected as little as 40 uU/mL glutamate oxidase in a 100 µL reaction volume. It can be performed in a convenient 96-well or 384-well microtiter-plate format and easily adapted to automation without a separation step.

References

View all 65 references: Citation Explorer

Structural characterization of L-glutamate oxidase from Streptomyces sp. X-119-6

Authors:

Arima J, Sasaki C, Sakaguchi C, Mizuno H, Tamura T, Kashima A, Kusakabe H, Sugio S, Inagaki K.

Journal:

Febs J (2009): 3894

Visualizing L-glutamate fluxes in acute hippocampal slices with glutamate oxidase-immobilized coverslips

Authors:

Okumura W, Moridera N, Kanazawa E, Shoji A, Hirano-Iwata A, Sugawara M.

Journal:

Anal Biochem (2009): 326

Coupling of energy metabolism and synaptic transmission at the transcriptional level: role of nuclear respiratory factor 1 in regulating both cytochrome c oxidase and NMDA glutamate receptor subunit genes

Authors:

Dhar SS, Wong-Riley MT.

Journal:

J Neurosci (2009): 483

Activation of metabotropic glutamate receptor 5 modulates microglial reactivity and neurotoxicity by inhibiting NADPH oxidase

Authors:

Loane DJ, Stoica BA, Pajoohesh-Ganji A, Byrnes KR, Faden AI.

Journal:

J Biol Chem (2009): 15629

Nuclear respiratory factor 1 co-regulates AMPA glutamate receptor subunit 2 and cytochrome c oxidase: tight coupling of glutamatergic transmission and energy metabolism in neurons

Authors:

Dhar SS, Liang HL, Wong-Riley MT.

Journal:

J Neurochem (2009): 1595

Spectral properties

Excitation (nm)	571
Emission (nm)	584

Usage and storage

Intended use

Research Use Only (RUO)

Instrument settings

Fluorescence microplate reader
Excitation	540 nm
Emission	590 nm
Cutoff	570 nm
Recommended plate	Solid black

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Page updated on October 6, 2026

Component A: Amplite® Red (light sensitive)	1 vial
Component B: Assay Buffer	1 bottle (20 mL)
Component C: Horseradish Peroxidase (lyophilized)	1 vial
Component D: Glutamic Acid	1 vial (3.4 mg)
Component E: Glutamate Oxidase Standard (lyophilized)	1 vial (15 mU, lyophilized)
Component F: DMSO	1 vial (200 µL)