Amplite® Fluorimetric Glutamate Oxidase Assay Kit *Red Fluorescence*

Amplite® Fluorimetric Glutamate Oxidase Assay Kit

Red Fluorescence

Glutamate oxidase belongs to the family of oxidoreductases, specifically those acting on the CH-NH2 group of donors with oxygen as an acceptor. It is an enzyme that specifically catalyzes the oxidative deamination of L-glutamate in the presence of water and oxygen with the formation of o-ketoglutarate, ammonia, and hydrogen peroxide. The Amplite® Fluorimetric Glutamate Oxidase Assay Kit provides a quick and ultrasensitive method for the measurement of glutamate oxidase in solution and in cell lysates. In the assay, L-glutamic acid is oxidized to µ-ketoglutarate, NH3 and H2O2 by glutamate oxidase. L-Alanine and L-glutamate-pyruvate transaminase are included in the reaction, resulting in multiple cycles of the initial reaction, thus significantly amplifying the production of H2O2. The kit uses our Amplite® Red substrate which enables a dual recordable mode. The fluorescent signal can be easily read by either a fluorescence microplate reader or an absorbance microplate reader. With the Amplite® Glutamate Oxidase Assay kit, we have detected as little as 40 uU/mL glutamate oxidase in a 100 µL reaction volume. It can be performed in a convenient 96-well or 384-well microtiter-plate format and easily adapted to automation without a separation step.

Glutamate oxidase dose response was measured with Amplite® Fluorimetric Glutamate Oxidase Assay Kit in a 96-well solid black plate using a Gemini fluorescence microplate reader (Molecular Devices).

Protocol

SDS

COA

Catalog	Size	Price	Quantity
11302	200 Tests	Price

References

View all 65 references: Citation Explorer

Structural characterization of L-glutamate oxidase from Streptomyces sp. X-119-6

Authors:

Arima J, Sasaki C, Sakaguchi C, Mizuno H, Tamura T, Kashima A, Kusakabe H, Sugio S, Inagaki K.

Journal:

Febs J (2009): 3894

Visualizing L-glutamate fluxes in acute hippocampal slices with glutamate oxidase-immobilized coverslips

Authors:

Okumura W, Moridera N, Kanazawa E, Shoji A, Hirano-Iwata A, Sugawara M.

Journal:

Anal Biochem (2009): 326

Coupling of energy metabolism and synaptic transmission at the transcriptional level: role of nuclear respiratory factor 1 in regulating both cytochrome c oxidase and NMDA glutamate receptor subunit genes

Authors:

Dhar SS, Wong-Riley MT.

Journal:

J Neurosci (2009): 483

Activation of metabotropic glutamate receptor 5 modulates microglial reactivity and neurotoxicity by inhibiting NADPH oxidase

Authors:

Loane DJ, Stoica BA, Pajoohesh-Ganji A, Byrnes KR, Faden AI.

Journal:

J Biol Chem (2009): 15629

Nuclear respiratory factor 1 co-regulates AMPA glutamate receptor subunit 2 and cytochrome c oxidase: tight coupling of glutamatergic transmission and energy metabolism in neurons

Authors:

Dhar SS, Liang HL, Wong-Riley MT.

Journal:

J Neurochem (2009): 1595

Spectral properties

Excitation (nm)	571
Emission (nm)	584

Storage, safety and handling

H-phrase	H303, H313, H333
Hazard symbol	XN
Intended use	Research Use Only (RUO)
R-phrase	R20, R21, R22
UNSPSC	12171501

Instrument settings

Fluorescence microplate reader
Excitation	540 nm
Emission	590 nm
Cutoff	570 nm
Recommended plate	Solid black

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Page updated on October 9, 2025

Component A: Amplite™ Red (light sensitive)	1 vial
Component B: Assay Buffer	1 bottle (20 mL)
Component C: Horseradish Peroxidase (lyophilized)	1 vial
Component D: Glutamic Acid	1 vial (3.4 mg)
Component E: Glutamate Oxidase Standard (lyophilized)	1 vial (15 mU, lyophilized)
Component F: DMSO	1 vial (200 µL)