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When should I use a TAE buffer for gel electrophoresis?
Posted April 26, 2023
Chemical ReagentsAgarose gel electrophoresisBuffer Preparations and RecipesGel Electrophoresisbuffer
Answer

TAE (Tris-acetate-EDTA) buffer produces better separation of fragments larger than 2 kb and is a good choice for gel electrophoresis when working with larger DNA fragments. It also works better for DNA extraction from agarose gel. 

TAE is better for cloning because, unlike TBE, it doesn’t contain borate, which is an inhibitor for many enzymes. 

Additional resources
The free solution mobility of DNA in Tris-acetate-EDTA buffers of different concentrations, with and without added NaCl Buffers and Lab Consumables TAE (1 M, pH 8.6) Preparation and Recipe

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