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When should I use a TBE buffer for gel electrophoresis?
Posted April 26, 2023
Chemical ReagentsAgarose gel electrophoresisBuffer Preparations and RecipesGel Electrophoresisbuffer
Answer

TBE (Tris-borate-EDTA) buffer is a good choice for gel electrophoresis when you require higher resolution of DNA fragments smaller than 2 kb. You’ll get sharper bands when you use TBE for smaller fragments. 

TBE also works better for a longer run because of its higher buffering capacity.

Additional resources
Modification of gel architecture and TBE/TAE buffer composition to minimize heating during agarose gel electrophoresis Buffers and Lab Consumables TAE (1 M, pH 8.6) Preparation and Recipe

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