Which DNA polymerase is most fidelity-oriented?
Posted August 15, 2024
Answer
DNA polymerase III is considered the most fidelity-oriented of the three polymerases because it facilitates precise DNA replication with fewer mistakes compared to DNA polymerase I and DNA polymerase II. This is because DNA polymerase III possesses a 3' to 5' exonuclease activity, which allows it to remove incorrect nucleotides immediately after they are added.
Additional resources
Fidelity of DNA replication—a matter of proofreading
Helixyte™ Green Fluorimetric Total Nucleic Acid Quantitation Kit *Optimized for Microplate Readers*