AAT Bioquest

Which metabolic pathway produces the most ATP?

Posted November 10, 2022


Oxidative phosphorylation is the metabolic pathway that produces the most ATP. It is a part of the cellular respiration process. The energy required to drive oxidative phosphorylation comes from oxidation-reduction reactions. There are a few other processes that also produce ATP but in much smaller quantities. Glycolysis and Krebs Cycle produce 2 ATP molecules per glucose molecule, while oxidative phosphorylation produces 28 ATP molecules per glucose molecule. 

Additional resources


PhosphoWorks™ Colorimetric ATP Assay Kit

ATP synthesis and storage