AAT Bioquest

Why is subculturing suspension cells easier and less complicated than passaging adherent cells?

Posted December 15, 2023


Subculturing suspension cells is easier compared to passaging adherent cells since these cells are already in a suspended state within the growth medium. There is no requirement to use enzymes to detach them from the culture vessel’s surface. This makes the entire process quicker and less stressful on the cells. Additionally, in suspension cultures, changing the growth medium is not necessary. Instead, cells are sustained by feeding them every 2-3 days until they cover the culture vessel. This is achieved by either directly diluting the cells within the flask to continue their growth, or by removing a portion of the cells and leaving the remaining cells at a suitable density for a specific cell line. 

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