FAQ
Posted on May 12, 2023
What are the differences between forward primer and reverse primer?
DNA and RNA QuantitationDNA and RNANucleic AcidsNucleusPCRPCR primerPCR stepsPolymerase Chain Reaction (PCR)Types of PCR
What are the differences between forward primer and reverse primer?
DNA and RNA QuantitationDNA and RNANucleic AcidsNucleusPCRPCR primerPCR stepsPolymerase Chain Reaction (PCR)Types of PCR
Posted on May 12, 2023
What is the principle of PCR?
DNA and RNA QuantitationDNA and RNANucleic AcidsNucleusPCRPCR primerPCR stepsPolymerase Chain Reaction (PCR)Types of PCR
What is the principle of PCR?
DNA and RNA QuantitationDNA and RNANucleic AcidsNucleusPCRPCR primerPCR stepsPolymerase Chain Reaction (PCR)Types of PCR
Posted on May 12, 2023
What are the similarities between primers and promotors?
DNA and RNA QuantitationDNA and RNANucleic AcidsNucleusPCR Detection of Viral DNA/RNAPCR primerPCR stepsPolymerase Chain Reaction (PCR)Real-Time PCR (qPCR)Reverse Transcription PCR (RT-PCR)Types of PCRgene sequencing
What are the similarities between primers and promotors?
DNA and RNA QuantitationDNA and RNANucleic AcidsNucleusPCR Detection of Viral DNA/RNAPCR primerPCR stepsPolymerase Chain Reaction (PCR)Real-Time PCR (qPCR)Reverse Transcription PCR (RT-PCR)Types of PCRgene sequencing
Posted on May 12, 2023
What are the differences between primers and promotors?
DNA and RNA QuantitationDNA and RNANucleic AcidsNucleusPCR Detection of Viral DNA/RNAPCR primerPCR stepsPolymerase Chain Reaction (PCR)Real-Time PCR (qPCR)Reverse Transcription PCR (RT-PCR)Types of PCR
What are the differences between primers and promotors?
DNA and RNA QuantitationDNA and RNANucleic AcidsNucleusPCR Detection of Viral DNA/RNAPCR primerPCR stepsPolymerase Chain Reaction (PCR)Real-Time PCR (qPCR)Reverse Transcription PCR (RT-PCR)Types of PCR
Posted on May 12, 2023
How does Real-Time PCR work?
DNA and RNA QuantitationDNA and RNANucleic AcidsNucleusPCRPCR Detection of Viral DNA/RNAPCR primerPCR stepsPolymerase Chain Reaction (PCR)Real-Time PCR (qPCR)Reverse Transcription PCR (RT-PCR)Types of PCR
How does Real-Time PCR work?
DNA and RNA QuantitationDNA and RNANucleic AcidsNucleusPCRPCR Detection of Viral DNA/RNAPCR primerPCR stepsPolymerase Chain Reaction (PCR)Real-Time PCR (qPCR)Reverse Transcription PCR (RT-PCR)Types of PCR
Posted on May 12, 2023
What are the advantages of Real-time PCR over PCR?
DNA and RNA QuantitationDNA and RNANucleic AcidsNucleusPCR Detection of Viral DNA/RNAPCR primerPCR stepsPolymerase Chain Reaction (PCR)Real-Time PCR (qPCR)Reverse Transcription PCR (RT-PCR)Types of PCR
What are the advantages of Real-time PCR over PCR?
DNA and RNA QuantitationDNA and RNANucleic AcidsNucleusPCR Detection of Viral DNA/RNAPCR primerPCR stepsPolymerase Chain Reaction (PCR)Real-Time PCR (qPCR)Reverse Transcription PCR (RT-PCR)Types of PCR
Posted on May 12, 2023
What should I consider when I set up a PCR efficiency test for my assay?
DNA and RNA QuantitationDNA and RNAPCRPCR Detection of Viral DNA/RNAPCR primerPCR stepsPolymerase Chain Reaction (PCR)Real-Time PCR (qPCR)Reverse Transcription PCR (RT-PCR)
What should I consider when I set up a PCR efficiency test for my assay?
DNA and RNA QuantitationDNA and RNAPCRPCR Detection of Viral DNA/RNAPCR primerPCR stepsPolymerase Chain Reaction (PCR)Real-Time PCR (qPCR)Reverse Transcription PCR (RT-PCR)
Posted on April 27, 2023
What happens if I add too much MgCI2 in my PCR reaction?
DNADNA and RNA QuantitationDNA and RNAPCRPCR primerPolymerase Chain Reaction (PCR)magnesium
What happens if I add too much MgCI2 in my PCR reaction?
DNADNA and RNA QuantitationDNA and RNAPCRPCR primerPolymerase Chain Reaction (PCR)magnesium
Posted on April 27, 2023
What happens if I add too little MgCI2 in my PCR reaction?
DNADNA and RNA QuantitationDNA and RNAEnzymesPCR primerPolymerase Chain Reaction (PCR)
What happens if I add too little MgCI2 in my PCR reaction?
DNADNA and RNA QuantitationDNA and RNAEnzymesPCR primerPolymerase Chain Reaction (PCR)
Posted on April 27, 2023
In PCR, what are the functions of DMSO?
DMSODNADNA and RNA QuantitationDNA and RNAPCRPCR primerPolymerase Chain Reaction (PCR)
In PCR, what are the functions of DMSO?
DMSODNADNA and RNA QuantitationDNA and RNAPCRPCR primerPolymerase Chain Reaction (PCR)
Posted on March 1, 2023
What are the common primers used in reverse transcription?
DNA and RNA QuantitationDNA and RNAPCR primerPeptide and OligonucleotidePolymerase Chain Reaction (PCR)RNAReverse Transcription PCR (RT-PCR)
What are the common primers used in reverse transcription?
DNA and RNA QuantitationDNA and RNAPCR primerPeptide and OligonucleotidePolymerase Chain Reaction (PCR)RNAReverse Transcription PCR (RT-PCR)
Posted on March 1, 2023
What are the reaction components for reverse transcription?
DNA and RNA QuantitationDNA and RNAEnzymesNucleic AcidsPCR Detection of Viral DNA/RNAPCR primerPolymerase Chain Reaction (PCR)Real-Time PCR (qPCR)Reverse Transcription PCR (RT-PCR)bufferdNTPsinhibitor
What are the reaction components for reverse transcription?
DNA and RNA QuantitationDNA and RNAEnzymesNucleic AcidsPCR Detection of Viral DNA/RNAPCR primerPolymerase Chain Reaction (PCR)Real-Time PCR (qPCR)Reverse Transcription PCR (RT-PCR)bufferdNTPsinhibitor
Posted on March 1, 2023
How do I optimize my reverse transcription process?
DNA and RNA QuantitationDNA and RNAGenomic DNANucleic AcidsPCR Detection of Viral DNA/RNAPCR primerPolymerase Chain Reaction (PCR)Real-Time PCR (qPCR)Reverse Transcription PCR (RT-PCR)
How do I optimize my reverse transcription process?
DNA and RNA QuantitationDNA and RNAGenomic DNANucleic AcidsPCR Detection of Viral DNA/RNAPCR primerPolymerase Chain Reaction (PCR)Real-Time PCR (qPCR)Reverse Transcription PCR (RT-PCR)
Posted on February 17, 2023
What happens if I use higher primer concentrations?
PCR Detection of Viral DNA/RNAPCR primerPolymerase Chain Reaction (PCR)Real-Time PCR (qPCR)Reverse Transcription PCR (RT-PCR)
What happens if I use higher primer concentrations?
PCR Detection of Viral DNA/RNAPCR primerPolymerase Chain Reaction (PCR)Real-Time PCR (qPCR)Reverse Transcription PCR (RT-PCR)
Posted on February 17, 2023
What happens if I only use a forward primer?
DNA and RNAPCR primerPolymerase Chain Reaction (PCR)
What happens if I only use a forward primer?
DNA and RNAPCR primerPolymerase Chain Reaction (PCR)
Posted on February 13, 2023
How do I find the concentration of a primer?
DNA and RNA QuantitationDNA and RNANucleic AcidsNucleusPCR Detection of Viral DNA/RNAPCR primerPolymerase Chain Reaction (PCR)
How do I find the concentration of a primer?
DNA and RNA QuantitationDNA and RNANucleic AcidsNucleusPCR Detection of Viral DNA/RNAPCR primerPolymerase Chain Reaction (PCR)
Posted on June 22, 2020
Why should primers used in PCR have similar melting temperatures?
DNAPCRPCR primerPolymerase Chain Reaction (PCR)ssDNA
Why should primers used in PCR have similar melting temperatures?
DNAPCRPCR primerPolymerase Chain Reaction (PCR)ssDNA
Posted on June 22, 2020
Should PCR primers be complementary to each other?
DNADNA and RNAPCRPCR primerPolymerase Chain Reaction (PCR)Polymerases
Should PCR primers be complementary to each other?
DNADNA and RNAPCRPCR primerPolymerase Chain Reaction (PCR)Polymerases
Posted on June 22, 2020
Why are 2 primers needed for PCR?
DNADNA and RNAPCRPCR primerPolymerase Chain Reaction (PCR)
Why are 2 primers needed for PCR?
DNADNA and RNAPCRPCR primerPolymerase Chain Reaction (PCR)
Posted on June 22, 2020
Why does PCR reaction require a primer?
DNADNA and RNAPCRPCR primerPolymerase Chain Reaction (PCR)Polymerases
Why does PCR reaction require a primer?
DNADNA and RNAPCRPCR primerPolymerase Chain Reaction (PCR)Polymerases
Posted on May 9, 2020
What are reverse transcription primers?
Antibodies and Proteomics Peptide and Oligonucleotide LabelingCellular ProcessesDNA and RNADNA and RNA QuantitationPeptide and OligonucleotideRNAPCR primer
What are reverse transcription primers?
Antibodies and Proteomics Peptide and Oligonucleotide LabelingCellular ProcessesDNA and RNADNA and RNA QuantitationPeptide and OligonucleotideRNAPCR primer
Posted on May 9, 2020
What are PCR primers?
Antibodies and ProteomicsDNA and RNAPCRPCR primerPolymerase Chain Reaction (PCR)Real-Time PCR (qPCR)Reverse Transcription PCR (RT-PCR)qPCR
What are PCR primers?
Antibodies and ProteomicsDNA and RNAPCRPCR primerPolymerase Chain Reaction (PCR)Real-Time PCR (qPCR)Reverse Transcription PCR (RT-PCR)qPCR
Posted on February 4, 2020
What is the difference between a PCR primer and a sequencing primer?
PCR primerSequencing primer
What is the difference between a PCR primer and a sequencing primer?
PCR primerSequencing primer