6-VIC Alkyne

Ordering information

Price
Catalog Number
Unit Size
Quantity

Add to cart

Additional ordering information

Telephone	1-800-990-8053
Fax	1-800-609-2943
Email	sales@aatbio.com
International	See distributors
Bulk request	Inquire
Custom size	Inquire
Shipping	Standard overnight for United States, inquire for international

Physical properties

Molecular weight	592.81
Solvent	DMSO

Spectral properties

Excitation (nm)	526
Emission (nm)	543

Storage, safety and handling

H-phrase	H303, H313, H333
Hazard symbol	XN
Intended use	Research Use Only (RUO)
R-phrase	R20, R21, R22
Storage	Freeze (< -15 °C); Minimize light exposure

Alternative formats

Overview SDS Protocol

Calculators

Common stock solution preparation

Table 1. Volume of DMSO needed to reconstitute specific mass of 6-VIC Alkyne to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

	0.1 mg	0.5 mg	1 mg	5 mg	10 mg
1 mM	168.688 µL	843.441 µL	1.687 mL	8.434 mL	16.869 mL
5 mM	33.738 µL	168.688 µL	337.376 µL	1.687 mL	3.374 mL
10 mM	16.869 µL	84.344 µL	168.688 µL	843.441 µL	1.687 mL

Molarity calculator

Enter any two values (mass, volume, concentration) to calculate the third.

Mass (Calculate)		Molecular weight		Volume (Calculate)		Concentration (Calculate)		Moles
	/		=		x		=

Spectrum

Open in Advanced Spectrum Viewer

Spectral properties

Excitation (nm)	526
Emission (nm)	543

Product Family

Name	Excitation (nm)	Emission (nm)	Extinction coefficient (cm ^-1 M ^-1)	Correction Factor (280 nm)
6-FAM Alkyne	493	517	83000	0.178
6-HEX alkyne	533	559	73000	0.15
6-TET alkyne	521	542	73000	0.13
6-JOE alkyne	520	545	75000¹	-
6-TAMRA alkyne	552	578	90000	0.178
6-ROX alkyne	578	604	82000	0.168
6-VIC, SE [6-VIC NHS ester]	526	543	-	-
6-NED alkyne	545	567	74000¹	-
6-VIC phosphoramidite	526	543	-	-

Images

Figure 1. 6-VIC alkyne is a fluorescent compound that contains an alkynyl group used in a variety of DNA sequencing and PCR applications. It is often used in combination with a complementary molecule bearing an azido group in a click reaction. This reaction forms a stable triazole linkage between the azido and alkyne groups, allowing for the covalent attachment of a variety of biomolecules, drugs, or imaging agents to a target molecule or surface.

References

View all 50 references: Citation Explorer

Genotoxicity assessment of textile waste contaminated soil and characterization of textile dye degradation by a novel indigenous bacterium Ochrobactrum intermedium BS39.
Authors: Khan, Sana and Zeyad, Mohammad Tarique and Malik, Abdul
Journal: Chemosphere (2022): 134082

Amplified Fluorescence in Situ Hybridization by Small and Bright Dye-Loaded Polymeric Nanoparticles.
Authors: Egloff, Sylvie and Melnychuk, Nina and Cruz Da Silva, Elisabete and Reisch, Andreas and Martin, Sophie and Klymchenko, Andrey S
Journal: ACS nano (2022): 1381-1394

Anomalies in dye-terminator DNA sequencing caused by a natural G-quadruplex.
Authors: Brush, George S
Journal: PloS one (2022): e0279423

Brevibacterium limosum sp. nov., Brevibacterium pigmenatum sp. nov., and Brevibacterium atlanticum sp. nov., three novel dye decolorizing actinobacteria isolated from ocean sediments.
Authors: Pei, Shengxiang and Niu, Siwen and Xie, Fuquan and Wang, Wenjing and Zhang, Shuang and Zhang, Gaiyun
Journal: Journal of microbiology (Seoul, Korea) (2021): 898-910

Mycoremediation of reactive red HE7B dye by Aspergillus salinarus isolated from textile effluents.
Authors: Sheam, Md Moinuddin and Biswas, Sudhangshu Kumar and Ahmed, Kazi Rejvee and Syed, Shifath Bin and Hossain, Md Shamim and Khan, Md Shakil Ahmed and Hasan, Md Rockybul and Zohra, Fatema Tuj and Rahman, Md Mizanur
Journal: Current research in microbial sciences (2021): 100056

Promiscuous dye binding by a light-up aptamer: application for label-free multi-wavelength biosensing.
Authors: Connelly, Ryan P and Madalozzo, Pedro F and Mordeson, Jack E and Pratt, Andrew D and Gerasimova, Yulia V
Journal: Chemical communications (Cambridge, England) (2021): 3672-3675

Construction of a Dye-Sensitized and Gold Plasmon-Enhanced Cathodic Photoelectrochemical Biosensor for Methyltransferase Activity Assay.
Authors: Cui, Lin and Shen, Jingzhu and Li, Chen-Chen and Cui, Pei-Pei and Luo, Xiliang and Wang, Xiaolei and Zhang, Chun-Yang
Journal: Analytical chemistry (2021): 10310-10316

Target sequence heterogeneity causes the 'hook effect' in fluorescent dye-based quantitative PCR.
Authors: Warton, Kristina and Xu, Yue and Ford, Caroline Elizabeth
Journal: BioTechniques (2020): 80-83

High Throughput Sediment DNA Sequencing Reveals Azo Dye Degrading Bacteria Inhabit Nearshore Sediments.
Authors: Zhuang, Mei and Sanganyado, Edmond and Xu, Liang and Zhu, Jianming and Li, Ping and Liu, Wenhua
Journal: Microorganisms (2020)

Genetic Diversity and Dye-Decolorizing Spectrum of Schizophyllum commune Population.
Authors: Choi, Yongjun and Nguyen, Ha Thi Kim and Lee, Tae Soo and Kim, Jae Kwang and Choi, Jaehyuk
Journal: Journal of microbiology and biotechnology (2020): 1525-1535