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ReadiUse™ Cell Detaching Buffer
Ordering information
| Price | |
| Catalog Number | |
| Unit Size | |
| Quantity |
Additional ordering information
| Telephone | 1-800-990-8053 |
| Fax | 1-800-609-2943 |
| sales@aatbio.com | |
| International | See distributors |
| Bulk request | Inquire |
| Custom size | Inquire |
| Shipping | Standard overnight for United States, inquire for international |
Storage, safety and handling
| H-phrase | H303, H313, H333 |
| Hazard symbol | XN |
| Intended use | Research Use Only (RUO) |
| R-phrase | R20, R21, R22 |
| Storage | Freeze (< -15 °C); Minimize light exposure |
| UNSPSC | 12352200 |
Related products
| Overview |  SDSProtocol |
See also: Buffers and Lab Consumables
ReadiUse™ cell detaching buffer is a cell detachment solution that does not contain mammalian or bacterial-derived products. It works similar to trypsin with much less toxic effect. It performs exceptionally well in detaching primary and stem cells and maintains high cell viability. ReadiUse™ cell detaching buffer is very useful for routine cell passage, analysis of cell surface markers and receptors, cell proliferation, apoptosis and flow cytometry.
Example protocol
SAMPLE EXPERIMENTAL PROTOCOL
Note:
The ReadiUse™ Cell Detaching Buffer is supplied as a sterile, ready-to use, frozen liquid. After thawing, It may be stored for up to 2 months at 4 °C.
The following protocol can be used as a guideline and can be modified as per user's need.
Sample protocol
Thaw the ReadiUse™ cell detaching buffer at room temperature.
Aspirate all of the media from the cell culture dish or flask. Note: It is not necessary to wash PBS.
Add the ReadiUse™ cell detaching buffer to the culture dish or flask to cover the cells, typically using ~10 mL for each 75 cm2 of surface area.
Return culture to 37 °C incubator and allow cells to detach for 5–10 minutes.
Count cells and passage as usual, no additional washes or enzyme inhibitors are required.
Images
Figure 1. Detaching of Hela cells with ReadiUse™ Cell Detaching Buffer.
Citations
View all 30 citations: Citation Explorer
A Simple Cell-based Immunofluorescence Assay to Detect Autoantibody Against the N-Methyl-D-Aspartate (NMDA) Receptor in Blood
Authors: Chen, C. H., Chang, Y. S.
Journal: J Vis Exp (2018): se name="60010.enl" path="C:\Website\Referenc
Authors: Chen, C. H., Chang, Y. S.
Journal: J Vis Exp (2018): se name="60010.enl" path="C:\Website\Referenc
The virtual cell based assay: Current status and future perspectives
Authors: Graepel, R., Lamon, L., Asturiol, D., Berggren, E., Joossens, E., Paini, A., Prieto, P., Whelan, M., Worth, A.
Journal: Toxicol In Vitro (2017): 258-267
Authors: Graepel, R., Lamon, L., Asturiol, D., Berggren, E., Joossens, E., Paini, A., Prieto, P., Whelan, M., Worth, A.
Journal: Toxicol In Vitro (2017): 258-267
Evaluation of anti-Zika virus activities of broad-spectrum antivirals and NIH clinical collection compounds using a cell-based, high-throughput screen assay
Authors: Adcock, R. S., Chu, Y. K., Golden, J. E., Chung, D. H.
Journal: Antiviral Res (2017): 47-56
Authors: Adcock, R. S., Chu, Y. K., Golden, J. E., Chung, D. H.
Journal: Antiviral Res (2017): 47-56
A cell-based high-throughput screening assay system for inhibitor compounds of antigen presentation by HLA class II molecule
Authors: Watanabe, N., Suzuki, Y., Yonezu, T., Nakagawa, Y., Shiina, T., Hirayama, N., Inokuchi, S., Inoue, S.
Journal: Sci Rep (2017): 6798
Authors: Watanabe, N., Suzuki, Y., Yonezu, T., Nakagawa, Y., Shiina, T., Hirayama, N., Inokuchi, S., Inoue, S.
Journal: Sci Rep (2017): 6798
Fluorescent Parkin Cell-Based Assay Development for the Screening of Drugs against Parkinson Disease
Authors: Villace, P., Mella, R. M., Roura-Ferrer, M., Valcarcel, M., Salado, C., Castilla, A., Kortazar, D.
Journal: SLAS Discov (2017): 67-76
Authors: Villace, P., Mella, R. M., Roura-Ferrer, M., Valcarcel, M., Salado, C., Castilla, A., Kortazar, D.
Journal: SLAS Discov (2017): 67-76
Establishment of Cell-Based Neuroglobin Promoter Reporter Assay for Neuroprotective Compounds Screening
Authors: Liu, N., Yu, Z., Gao, X., Song, Y. S., Yuan, J., Xun, Y., Wang, T., Yan, F., Yuan, S., Zhang, J., Xiang, S., Lo, E. H., Wang, X.
Journal: CNS Neurol Disord Drug Targets (2016): 629-39
Authors: Liu, N., Yu, Z., Gao, X., Song, Y. S., Yuan, J., Xun, Y., Wang, T., Yan, F., Yuan, S., Zhang, J., Xiang, S., Lo, E. H., Wang, X.
Journal: CNS Neurol Disord Drug Targets (2016): 629-39
A cell-based screening assay to identify pharmaceutical compounds that enhance the regenerative quality of corneal repair
Authors: Gordon, G. M., LaGier, A. J., Ponchel, C., Bauskar, A., Itakura, T., Jeong, S., Patel, N., Fini, M. E.
Journal: Wound Repair Regen (2016): 89-99
Authors: Gordon, G. M., LaGier, A. J., Ponchel, C., Bauskar, A., Itakura, T., Jeong, S., Patel, N., Fini, M. E.
Journal: Wound Repair Regen (2016): 89-99
Cell-Based Assay for Identifying the Modulators of Antioxidant Response Element Signaling Pathway
Authors: Zhao, J., Shukla, S. J., Xia, M.
Journal: Methods Mol Biol (2016): 55-62
Authors: Zhao, J., Shukla, S. J., Xia, M.
Journal: Methods Mol Biol (2016): 55-62
Validation of a human cell based high-throughput genotoxicity assay 'Anthem's Genotoxicity screen' using ECVAM recommended lists of genotoxic and non-genotoxic chemicals
Authors: Rajakrishna, L., Krishnan Unni, S., Subbiah, M., Sadagopan, S., Nair, A. R., Ch and rappa, R., Sambasivam, G., Sukumaran, S. K.
Journal: Toxicol In Vitro (2014): 46-53
Authors: Rajakrishna, L., Krishnan Unni, S., Subbiah, M., Sadagopan, S., Nair, A. R., Ch and rappa, R., Sambasivam, G., Sukumaran, S. K.
Journal: Toxicol In Vitro (2014): 46-53
Development of a novel cell-based assay system EPISSAY for screening epigenetic drugs and liposome formulated decitabine
Authors: Lim, S. P., Kumar, R., Akkamsetty, Y., Wang, W., Ho, K., Neilsen, P. M., Walther, D. J., Suetani, R. J., Prestidge, C., Callen, D. F.
Journal: BMC Cancer (2013): 113
Authors: Lim, S. P., Kumar, R., Akkamsetty, Y., Wang, W., Ho, K., Neilsen, P. M., Walther, D. J., Suetani, R. J., Prestidge, C., Callen, D. F.
Journal: BMC Cancer (2013): 113