ReadiUse™ MTS Reagent 5 mM aqueous solution

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Additional ordering information

Telephone	1-800-990-8053
Fax	1-800-609-2943
Email	sales@aatbio.com
International	See distributors
Bulk request	Inquire
Custom size	Inquire
Shipping	Standard overnight for United States, inquire for international

Physical properties

Molecular weight	487.51
Solvent	Water

Storage, safety and handling

H-phrase	H303, H313, H333
Hazard symbol	XN
Intended use	Research Use Only (RUO)
R-phrase	R20, R21, R22
Storage	Freeze (< -15 °C); Minimize light exposure

Overview SDS Protocol

CAS

138169-43-4

Molecular weight

487.51

MTS reagent is one of the most common water-soluble tetrazolium reagents that are widely used to quantify cell viability by absorption spectrophotometry. A variety of tetrazolium inner salts are used for determining cell viability in proliferation assays by monitoring their color changes. Metabolically active cells reduce colorless tetrazolium inner salts into the colored formazan products that might be either soluble or insoluble in cell culture media depending on the structures of tetrazolium reagents. The conversion of tetrazolium inner salts to formazans is thought to be carried out by NAD(P)H-dependent dehydrogenase enzymes.

Platform

Absorbance microplate reader

Absorbance	490 nm
Recommended plate	Clear bottom

Calculators

Common stock solution preparation

Table 1. Volume of Water needed to reconstitute specific mass of ReadiUse™ MTS Reagent *5 mM aqueous solution* to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

	0.1 mg	0.5 mg	1 mg	5 mg	10 mg
1 mM	205.124 µL	1.026 mL	2.051 mL	10.256 mL	20.512 mL
5 mM	41.025 µL	205.124 µL	410.248 µL	2.051 mL	4.102 mL
10 mM	20.512 µL	102.562 µL	205.124 µL	1.026 mL	2.051 mL

Molarity calculator

Enter any two values (mass, volume, concentration) to calculate the third.

Mass (Calculate)		Molecular weight		Volume (Calculate)		Concentration (Calculate)		Moles
	/		=		x		=

Images

Figure 1. Chemical structure of ReadiUse™ MTS Reagent.

References

View all 39 references: Citation Explorer

The edge effect: A global problem. The trouble with culturing cells in 96-well plates.
Authors: Mansoury, Morva and Hamed, Maya and Karmustaji, Rashid and Al Hannan, Fatima and Safrany, Stephen T
Journal: Biochemistry and biophysics reports (2021): 100987

Profiling the Neoplasm Microenvironment of Silica Nanomaterial-Derived Scaffolds of Single, 2-, and 3-Composite Systems.
Authors: Akpe, Victor and Murhekar, Shweta and Kim, Tak H and Brown, Christopher L and Cock, Ian E
Journal: Assay and drug development technologies (2021): 191-203

A Modified MTS Proliferation Assay for Suspended Cells to Avoid the Interference by Hydralazine and β-Mercaptoethanol.
Authors: Wang, Yutang and Nguyen, Dinh Tam and Yang, Guang and Anesi, Jack and Kelly, Jason and Chai, Zhonglin and Ahmady, Fahima and Charchar, Fadi and Golledge, Jonathan
Journal: Assay and drug development technologies (2021): 184-190

Synthesis, structural characterization, and prospects for new cobalt (II) complexes with thiocarbamoyl-pyrazoline ligands as promising antifungal agents.
Authors: Dias, Bianca Boni and da Silva Dantas, Fabiana Gomes and Galvão, Fernanda and Cupozak-Pinheiro, Wellinton Jhon and Wender, Heberton and Pizzuti, Lucas and Rosa, Persiely Pires and Tenório, Kátia Veronica and Gatto, Claudia Cristina and Negri, Melyssa and Casagrande, Gleison Antônio and de Oliveira, Kelly Mari Pires
Journal: Journal of inorganic biochemistry (2020): 111277

The Predictive Value of Estrogen Receptor 1 on Adjuvant Chemotherapy in Locally Advanced Colorectal Cancer: A Retrospective Analysis With Independent Validation and Its Potential Mechanism.
Authors: Ye, Shu-Biao and Cheng, Yi-Kan and Deng, Ru and Deng, Yanhong and Li, Peisi and Zhang, Lin and Lan, Ping
Journal: Frontiers in oncology (2020): 214

Calcineurin/NFAT Signaling Modulates Pulmonary Artery Smooth Muscle Cell Proliferation, Migration and Apoptosis in Monocrotaline-Induced Pulmonary Arterial Hypertension Rats.
Authors: He, Rui-Lan and Wu, Zhi-Juan and Liu, Xiao-Ru and Gui, Long-Xin and Wang, Rui-Xing and Lin, Mo-Jun
Journal: Cellular physiology and biochemistry : international journal of experimental cellular physiology, biochemistry, and pharmacology (2018): 172-189

Glutathione release through connexin hemichannels: Implications for chemical modification of pores permeable to large molecules.
Authors: Tong, Xuhui and Lopez, William and Ramachandran, Jayalakshmi and Ayad, Wafaa A and Liu, Yu and Lopez-Rodriguez, Angelica and Harris, Andrew L and Contreras, Jorge E
Journal: The Journal of general physiology (2015): 245-54

Caution is required in interpretation of mutations in the voltage sensing domain of voltage gated channels as evidence for gating mechanisms.
Authors: Kariev, Alisher M and Green, Michael E
Journal: International journal of molecular sciences (2015): 1627-43

Cysteine accessibility probes timing and extent of NBD separation along the dimer interface in gating CFTR channels.
Authors: Chaves, Luiz A Poletto and Gadsby, David C
Journal: The Journal of general physiology (2015): 261-83

CHARMM-GUI PDB manipulator for advanced modeling and simulations of proteins containing nonstandard residues.
Authors: Jo, Sunhwan and Cheng, Xi and Islam, Shahidul M and Huang, Lei and Rui, Huan and Zhu, Allen and Lee, Hui Sun and Qi, Yifei and Han, Wei and Vanommeslaeghe, Kenno and MacKerell, Alexander D and Roux, Benoît and Im, Wonpil
Journal: Advances in protein chemistry and structural biology (2014): 235-65