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ReadiUse™ Preactivated PerCP-Cy7 Maleimide
Ordering information
| Price | |
| Catalog Number | |
| Unit Size | |
| Quantity |
Additional ordering information
| Telephone | 1-800-990-8053 |
| Fax | 1-800-609-2943 |
| sales@aatbio.com | |
| International | See distributors |
| Bulk request | Inquire |
| Custom size | Inquire |
| Shipping | Standard overnight for United States, inquire for international |
Physical properties
| Molecular weight | N/A |
| Solvent | Water |
Spectral properties
| Extinction coefficient (cm -1 M -1) | 350000 |
| Excitation (nm) | 757 |
| Emission (nm) | 783 |
Storage, safety and handling
| H-phrase | H303, H313, H333 |
| Hazard symbol | XN |
| Intended use | Research Use Only (RUO) |
| R-phrase | R20, R21, R22 |
| Storage | Refrigerated (2-8 °C); Minimize light exposure |
Related products
| Overview |  SDSProtocol |
See also: PE and APC
Molecular weight N/A | Extinction coefficient (cm -1 M -1) 350000 | Excitation (nm) 757 | Emission (nm) 783 |
PerCP-Cy7 is a popular tandem color used in flow cytometry. Its primary absorption peak is at ~480 nm with emission peak at ~780 nm. AAT Bioquest offers this preactivated PerCP-Cy7 to facilitate the PerCP-Cy7 tandem conjugations to reduced antibodies and other biomolecules that contain a thiol group. Our preactivated PerCP-Cy7 maleimide is prepared from the commonly used crosslinker SMCC, and ready to conjugate. PerCP (Peridinin-chlorophyll-protein complex) is isolated from Dinophyceae sp. It has an extremely high extinction coefficient, a high quantum efficiency and a large Stokes shift. Its tandem conjugates (such as PerCP-Cy7) can be excited with a standard 488 nm laser and emits in the far red at a longer wavelength for multicolor flow cytometric analysis of cells. These multiple emission wavelengths make PerCP- Cyanine conjugates potentially useful fluorochromes for multicolor analysis with FITC, PE and other fluorochromes. PerCP tandem structure may make it more photostable than PerCP alone, which generally photobleaches rapidly with more powerful water-cooled gas lasers.
Spectrum
Open in Advanced Spectrum Viewer
Spectral properties
| Extinction coefficient (cm -1 M -1) | 350000 |
| Excitation (nm) | 757 |
| Emission (nm) | 783 |
Product Family
| Name | Excitation (nm) | Emission (nm) | Extinction coefficient (cm -1 M -1) |
| ReadiUse™ Preactivated PE-Cy7 Maleimide | 565 | 778 | 1960000 |
| ReadiUse™ Preactivated APC-Cy7 Maleimide | 651 | 779 | 700000 |
References
View all 15 references: Citation Explorer
Recombinant thrombomodulin attenuates hyper-inflammation and glycocalyx damage in a murine model of Streptococcus pneumoniae-induced sepsis.
Authors: Watanabe, Eizo and Akamatsu, Toshinobu and Ohmori, Masaaki and Kato, Mayu and Takeuchi, Noriko and Ishiwada, Naruhiko and Nishimura, Rintaro and Hishiki, Haruka and Fujimura, Lisa and Ito, Chizuru and Hatano, Masahiko
Journal: Cytokine (2022): 155723
Authors: Watanabe, Eizo and Akamatsu, Toshinobu and Ohmori, Masaaki and Kato, Mayu and Takeuchi, Noriko and Ishiwada, Naruhiko and Nishimura, Rintaro and Hishiki, Haruka and Fujimura, Lisa and Ito, Chizuru and Hatano, Masahiko
Journal: Cytokine (2022): 155723
Routine flow cytometry approach for the evaluation of solid tumor neoplasms and immune cells in minimally invasive samples.
Authors: Quirós-Caso, Covadonga and Arias Fernández, Tamara and Fonseca-Mourelle, Ariana and Torres, Héctor and Fernández, Luis and Moreno-Rodríguez, Maria and Ariza-Prota, Miguel Ángel and López-González, Francisco Julián and Carvajal-Álvarez, Miguel and Alonso-Álvarez, Sara and Moro-García, Marco Antonio and Colado, Enrique
Journal: Cytometry. Part B, Clinical cytometry (2022)
Authors: Quirós-Caso, Covadonga and Arias Fernández, Tamara and Fonseca-Mourelle, Ariana and Torres, Héctor and Fernández, Luis and Moreno-Rodríguez, Maria and Ariza-Prota, Miguel Ángel and López-González, Francisco Julián and Carvajal-Álvarez, Miguel and Alonso-Álvarez, Sara and Moro-García, Marco Antonio and Colado, Enrique
Journal: Cytometry. Part B, Clinical cytometry (2022)
The ISCCA flow protocol for the monitoring of anti-CD20 therapies in autoimmune disorders.
Authors: Gatti, Arianna and Buccisano, Francesco and Scupoli, Maria T and Brando, Bruno
Journal: Cytometry. Part B, Clinical cytometry (2021): 194-205
Authors: Gatti, Arianna and Buccisano, Francesco and Scupoli, Maria T and Brando, Bruno
Journal: Cytometry. Part B, Clinical cytometry (2021): 194-205
[Clinical features of imbalance between Th1 and Th22 cells and its association with disease progression in patients with liver cirrhosis].
Authors: Wu, H Q and Zhao, J J and Li, H W and Zhang, Z
Journal: Zhonghua gan zang bing za zhi = Zhonghua ganzangbing zazhi = Chinese journal of hepatology (2017): 738-744
Authors: Wu, H Q and Zhao, J J and Li, H W and Zhang, Z
Journal: Zhonghua gan zang bing za zhi = Zhonghua ganzangbing zazhi = Chinese journal of hepatology (2017): 738-744
Flow cytometric analysis of T lymphocyte proliferation in vivo by EdU incorporation.
Authors: Sun, Xiaojing and Zhang, Chunpan and Jin, Hua and Sun, Guangyong and Tian, Yue and Shi, Wen and Zhang, Dong
Journal: International immunopharmacology (2016): 56-65
Authors: Sun, Xiaojing and Zhang, Chunpan and Jin, Hua and Sun, Guangyong and Tian, Yue and Shi, Wen and Zhang, Dong
Journal: International immunopharmacology (2016): 56-65
Inhibition of histone deacetylase 6 restores innate immune cells in the bone marrow in a lethal septic model.
Authors: Zhao, Ting and Li, Yongqing and Liu, Baoling and Pan, Baihong and Cheng, Xin and Georgoff, Patrick and Alam, Hasan B
Journal: The journal of trauma and acute care surgery (2016): 34-40; discussion 40-1
Authors: Zhao, Ting and Li, Yongqing and Liu, Baoling and Pan, Baihong and Cheng, Xin and Georgoff, Patrick and Alam, Hasan B
Journal: The journal of trauma and acute care surgery (2016): 34-40; discussion 40-1
[THE STANDARD VALUES OF SUB-POPULATIONS OF T-HELPERS OF DIFFERENT LEVEL OF DIFFERENTIATION IN PERIPHERAL BLOOD].
Authors: Kudryavtsev, I V and Serebryakova, M K and Totolyan, A A
Journal: Klinicheskaia laboratornaia diagnostika (2016): 179-84
Authors: Kudryavtsev, I V and Serebryakova, M K and Totolyan, A A
Journal: Klinicheskaia laboratornaia diagnostika (2016): 179-84
Presence of CD34(+)CD38(-)CD58(-) leukemia-propagating cells at diagnosis identifies patients at high risk of relapse with Ph chromosome-positive ALL after allo-hematopoietic SCT.
Authors: Kong, Y and Xu, L-P and Liu, Y-R and Qin, Y-Z and Sun, Y-Q and Wang, Y and Jiang, H and Jiang, Q and Chen, H and Chang, Y-J and Huang, X-J
Journal: Bone marrow transplantation (2015): 348-53
Authors: Kong, Y and Xu, L-P and Liu, Y-R and Qin, Y-Z and Sun, Y-Q and Wang, Y and Jiang, H and Jiang, Q and Chen, H and Chang, Y-J and Huang, X-J
Journal: Bone marrow transplantation (2015): 348-53
Using six-colour flow cytometry to analyse the activation and interaction of platelets and leukocytes--A new assay suitable for bench and bedside conditions.
Authors: Granja, Tiago and Schad, Jessica and Schüssel, Patricia and Fischer, Claudius and Häberle, Helene and Rosenberger, Peter and Straub, Andreas
Journal: Thrombosis research (2015): 786-96
Authors: Granja, Tiago and Schad, Jessica and Schüssel, Patricia and Fischer, Claudius and Häberle, Helene and Rosenberger, Peter and Straub, Andreas
Journal: Thrombosis research (2015): 786-96
Combined normal donor and CLL: Single tube ZAP-70 analysis.
Authors: Degheidy, Heba A and Venzon, David J and Farooqui, Mohammed Z H and Abbasi, Fatima and Arthur, Diane C and Wilson, Wyndham H and Wiestner, Adrian and Stetler-Stevenson, M A and Marti, Gerald E
Journal: Cytometry. Part B, Clinical cytometry (2012): 67-77
Authors: Degheidy, Heba A and Venzon, David J and Farooqui, Mohammed Z H and Abbasi, Fatima and Arthur, Diane C and Wilson, Wyndham H and Wiestner, Adrian and Stetler-Stevenson, M A and Marti, Gerald E
Journal: Cytometry. Part B, Clinical cytometry (2012): 67-77
Application notes
A New Protein Crosslinking Method for Labeling and Modifying Antibodies
A Novel Fluorescent Probe for Imaging and Detecting Hydroxyl Radical in Living Cells
Buccutite™ Bioconjugation Technology
Monitoring of Mitochondrial Membrane Potential Changes in Live Cells Using JC-10
Multiplexing Cell Proliferation and Cytotoxicity Assays Using Calcein Red and CytoCalceins
A Novel Fluorescent Probe for Imaging and Detecting Hydroxyl Radical in Living Cells
Buccutite™ Bioconjugation Technology
Monitoring of Mitochondrial Membrane Potential Changes in Live Cells Using JC-10
Multiplexing Cell Proliferation and Cytotoxicity Assays Using Calcein Red and CytoCalceins
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Can I intracellularly measure mitochondria calcium flux and changes in mitochondria membrane potential at the same time?
Do you have fixable live cell staining assay kits?