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ALP Conjugates
Alkaline phosphatase (ALP) is a widely used enzyme reporter in immunoassays including ELISA, Western blotting, and molecular biology applications. ALP generates signal through colorimetric, fluorimetric, or chemiluminescent substrates, offering excellent sensitivity and low background.
AAT Bioquest provides ready-to-use ALP conjugates, antibody labeling kits, and purified enzyme for custom conjugation.
Key Advantages of ALP as a Reporter
- Compatible with multiple detection modes (colorimetric, fluorescent, luminescent)
- Stable enzyme with long shelf life
- Lower background due to less endogenous enzymes compared to peroxidases
- Greater signal stability, with readouts available hours later without significant decay
- Ideal for alkaline environments, with optimal function at pH 9 to 10
Ready to Use ALP Streptavidin Conjugate
AAT Bioquest offers pre-conjugated streptavidin-ALP, which is optimized for biotin-streptavidin detection systems. It can be used with any biotinylated primary antibody or probe for immunoassays, ELISA, and blotting applications.
Buccutite™ ALP Antibody Conjugation Kits
Buccutite™ labeling technology enables rapid, high-yield ALP conjugation to antibodies under mild conditions. Unlike traditional SMCC crosslinking, Buccutite™ uses pre-activated ALP that reacts with MTA-modified antibodies without catalysts, minimizing protein aggregation and activity loss.
Key Advantages
- Minimal optimization required, works with most IgG antibodies
- Mild reaction conditions preserve antibody activity
- Higher conjugation efficiency than SMCC methods
- Complete kit includes all reagents needed
For labeling proteins with other reporters, see our complete Buccutite™ conjugation kit collection.
Purified Alkaline Phosphatase Enzyme
AAT Bioquest offers calf-intestinal alkaline phosphatase (CIAP) for molecular biology applications and custom conjugation workflows. CIAP catalyzes dephosphorylation of DNA/RNA 5´ and 3´ ends and hydrolyzes nucleoside triphosphates.
Common Applications
- Dephosphorylation of linearized plasmid DNA to prevent self-ligation
- Removal of 5´ phosphates before end-labeling
- Degradation of unincorporated dNTPs in PCR cleanup
- Custom enzyme conjugate preparation for research applications where alkaline phosphatase activity is required
This document (01.0208.251203r1) was last updated on Thu Feb 12 2026. All trademarks and registered trademarks mentioned herein are the property of their respective owners.