Accurate detection and quantification of nucleic acids is fundamental to molecular biology, from basic research to clinical diagnostics. AAT Bioquest provides comprehensive reagents for DNA and RNA analysis, including fluorescent dyes for gel electrophoresis, quantification assays compatible with microplate readers and Qubit™/Cytocite™ instruments, and specialized reagents for PCR applications.

DNA quantification reagents enable precise measurement of double-stranded DNA, single-stranded DNA, and total nucleic acids using fluorescence-based detection. These assays offer superior sensitivity and specificity compared to UV absorbance methods, with detection limits in the picogram range.

DNA gel stains enable visualization of nucleic acids in agarose and polyacrylamide gels following electrophoresis. Our portfolio includes classic intercalating dyes and proprietary alternatives with improved sensitivity and safety profiles.

Gelite™ Safe DNA stains are non-mutagenic alternatives to ethidium bromide and GelRed for nucleic acid gel staining. These dyes provide comparable or superior sensitivity while eliminating hazardous waste disposal requirements.

SYBR stains and equivalents are asymmetric cyanine dyes widely used for nucleic acid detection in gel electrophoresis, flow cytometry, and qPCR applications. AAT Bioquest offers SYBR-equivalent dyes and calibration reagents for qPCR instrument validation.

RNA quantification reagents provide sensitive and selective measurement of RNA concentration using fluorescence-based detection. These assays are essential for preparing samples for downstream applications including RT-PCR, RNA-seq, and Northern blotting.

qPCR reagents include calibration solutions, reference dyes, and specialized building blocks for probe synthesis. These products ensure accurate instrument calibration and reliable quantitative PCR results across different platforms.

RT-PCR reagents enable reverse transcription of RNA templates into complementary DNA (cDNA) for subsequent PCR amplification. These kits provide the enzymes and buffers necessary for efficient first-strand cDNA synthesis.