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Secondary and Tag Antibodies
AAT Bioquest offers a comprehensive selection of secondary antibodies conjugated to iFluor® fluorescent dyes, phycobiliproteins (PE, APC, PerCP), and enzyme tags (HRP, ALP) for immunodetection applications. Our highly cross-adsorbed secondary antibodies minimize species cross-reactivity for clean multicolor experiments. We also provide a diverse collection of anti-tag molecules that enable detection and purification of common protein fusion tags including His, FLAG, HA, and GST.
Looking to label your own antibodies? Explore our ReadiLink™ Protein Labeling Kits for simple conjugation workflows.
Browse Secondary and Tag Antibodies
Anti-human IgG secondary antibodies detect human primary antibodies in immunoassays.
- iFluor® conjugates spanning UV to near-infrared wavelengths
- PE and APC conjugates for flow cytometry
- HRP and ALP conjugates for ELISA and Western blot
- Biotin conjugates for streptavidin-based amplification
- Cross-adsorbed against mouse, rabbit, and other species
Anti-rabbit IgG secondary antibodies provide sensitive detection of rabbit primary antibodies across all immunodetection platforms.
- Full iFluor® spectral range (350-790 nm) for microscopy
- PE, APC, and tandem conjugates for flow cytometry
- HRP conjugates for Western blot and ELISA
- F(ab')₂ fragments for reduced Fc receptor binding
- Cross-adsorbed for multiplexed experiments
Anti-mouse IgG secondary antibodies are our most comprehensive secondary antibody offering, supporting detection of mouse primary antibodies in all applications.
- Complete iFluor® series for multicolor immunofluorescence
- Phycobiliprotein conjugates (PE, APC, PerCP) for flow cytometry
- Enzyme conjugates (HRP, ALP) for chromogenic detection
- IgG subclass-specific options (IgG1, IgG2a, IgG2b, IgG3)
- Light chain specific (kappa, lambda) for specialized applications
Anti-tag detection reagents enable direct detection of recombinant fusion proteins bearing defined affinity tags and are commonly used in Western blotting, immunoprecipitation, pull-down assays, and immunofluorescence without reliance on secondary antibody amplification.
- Anti-His tag antibodies for polyhistidine-tagged proteins
- Anti-FLAG antibodies for FLAG epitope detection
- Anti-HA antibodies for hemagglutinin tag detection
- Anti-GST antibodies for glutathione S-transferase fusions
- Available with iFluor®, HRP, and biotin conjugates
This document (01.0285.251203r1) was last updated on Sat Feb 28 2026. All trademarks and registered trademarks mentioned herein are the property of their respective owners.