5-FITC [Fluorescein-5-isothiocyanate] *CAS 3326-32-7*

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Chemical structure for 5-FITC [Fluorescein-5-isothiocyanate] *CAS 3326-32-7*
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Overview

Ex/Em (nm)492/515
MW389.38
CAS #3326-32-7
SolventDMSO
Storage Freeze (<-15 °C)
Minimize light exposure
Category Peptide Synthesis
Peptide Labeling Reagents
Related Fluoresceins
Amine-Reactive Probes
5-FITC and 6-FITC have very similar absorption and fluorescence spectra. However, the isomers may differ in their binding and reactivities to proteins, and the conjugates may migrate differently in electrophoretic gels. Thus, we offer highly purified single isomers. Cautions must be exercised for the storage of FITC conjugates.




Calculators
Common stock solution preparation

Table 1. Volume of DMSO needed to reconstitute specific mass of 5-FITC [Fluorescein-5-isothiocyanate] *CAS 3326-32-7* to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.



Molarity calculator

Table 2. Enter any two values (mass, volume, concentration) to calculate the third.

Mass Molecular weight Volume Concentration Moles
/ = x =
 






Spectrum Advanced Spectrum Viewer

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Wavelength (nm)





References & Citations

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Journal: Journal of Biochemistry (2016): mvw040

Determination of sodium benzoate in food products by fluorescence polarization immunoassay
Authors: Linlin Ren, Meng Meng, Peng Wang, Zhihuan Xu, Sergei A Eremin, Junhong Zhao, Yongmei Yin, Rimo Xi
Journal: Talanta (2014): 136--143

Complexes containing cationic and anionic pH-sensitive liposomes: comparative study of factors influencing plasmid DNA gene delivery to tumors
Authors: Yan Chen, Ji Sun, Ying Lu, Chun Tao, Jingbin Huang, He Zhang, Yuan Yu, Hao Zou, Jing Gao, Yanqiang Zhong
Journal: International journal of nanomedicine (2013): 1573

Swimming behavior of the monotrichous bacterium Pseudomonas fluorescens SBW25
Authors: Liyan Ping, Jan Birkenbeil, Shamci Monajembashi
Journal: FEMS microbiology ecology (2013): 36--44






Additional Documents

 
Safety Data Sheet (SDS)


Catalogs
1. Oligonucleotide Labeling Reagents
2. Peptide Labeling Reagents

Certificate of Analysis